Fluorescence Polarization/Anisotropy Imaging with Zeiss Microscopes

LSM 780 - Obtain Quantitative Information About Your Cells and Individual Molecules. The sensitivity of LSM 780 is quite simply outstanding. The GaAsP detector achieves 45 percent quantum efficiency compared to 25 percent typically by conventional PMT detectors. This results in accurate details and contrast-rich images of the challenging specimens you encounter in your live cell imaging.The system’s illumination and detection design allows you to simultaneously acquire up to ten dyes. You excite any common fluorophore with up to eight different lasers, detecting the signals with the 32 channel GaAsP detector. LSM 780 is so sensitive, the system even allows photon counting.The maximum possible acquisition speed available to you is determined by the sensitivity of your detection system. When you choose LSM 780, you benefit from new GaAsP detectors that bring a significant improvement on signal-to-noise ratio, allowing you to acquire your image sequences at least twice as fast.Parallel spectral detection also reads 34 channels simultaneously in Lambda mode: you acquire and separate up to ten colors simultaneously.LSM 780 allows you to achieve images of unparalleled detail, even from the most challenging specimens. The superior quality and sensitivity has been recognized by a distinguished R&D 100 Award in 2011.LSM 780 Laser Scanning Microscope Features: 32-channel GaAsP detector with a typical quantum efficiency of 45% – a significant improvement on the 25% typically achieved by classic PMT detectors. Potential for photon counting on GaAsP and the new side PMT. With lasers from 355 nm true UV to 660 nm far red, any dye will be excited optimally. Simple adaptation to changing acquisition strategies via the ZEN software’s Smart Setup. Improved ZEN software with modules for e.g. correlative microscopy, deconvolution and time lapse. Improved dynamics for better visualization of weak signals. Active cooling together with oversampling and photon counting modes deliver improved signal-to-noise ratio.

Versatile measurements in solutions and cells: concentrations, interactions, fluctuations, diffusions. ConfoCor 3 is an ideal choice for single molecule imaging applications. It measures precisely with highest sensitivity and quickly within seconds. Based on the confocal detection principle, the diffusion speed and concentration of a fluorescent labeled molecule is easily accessed in a single measurement by the correlation function. No compromise in sensitivityThe new C-Apochromat 40x / W 1.2 (UV-VIS-IR) high numerical aperture objective guarantees a maximum collection efficiency of photons emitted by an excited molecule. These photons are detected with highest sensitivity by Avalanche photodiodes (APDs). Rely on efficient colour correction for best cross-correlation results. Marriage of Optics and Mathematics The confocal volume is produced by focusing the laser beam on the physically smallest possible spot usinghigh precision optics. Recorded fluctuations in fluorescence are then analyzed by a sophisticated mathematical equation, the correlation function. Automation to enhance your comfortConfoCor 3 is the fully motorized and computer controlled single molecule imaging solution. This microscope enables the automation of measurement protocols. High reproducibility in your measurements, high sample throughput and delegation of routine measurements go without saying.

LSM 700 rivals high end confocals with superb image quality Perform fluorescence measurements, create optical sections of your samples and combine them into 3D image stacks. ZEN imaging software shortens the training period and stores the settings for all users in your team. Smart Setup lets you configure new experiments easily with optimized recording settings. The compact design of LSM 700 means you will always have room for it in your work environment. Combine your LSM 700 with the upright microscope stand Axio Imager 2, inverted Axio Observer or the fixed stage microscope Axio Examiner for complete flexibility in your live cell imaging or photomanipulation applications.