ResourceLife Sciences
Flow Cytometry Analysis of Transcription Factor Expression During Differentiation of hPSC-Derived Cardiomyocytes
12 Sept 2017The ability to direct human pluripotent stem cells (hPSCs) toward differentiated cell phenotypes offers tremendous potential for personalized and regenerative medicine. The identification of key transcriptional regulators of pluripotency, as well as chemically defined media and cell culture conditions that drive PSCs towards distinct cell fates, have enabled researchers to derive a multitude of differentiated cell types with a high degree of control and precision. In this application note the Attune Nxt Flow Cytometer is demonstrated to enable single-cell quantification of cells expressing markers of both pluripotency and cardiomyocyte specification.
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Invitrogen™ Attune™ Flow Cytometer - Blue/Red Lasers
Thermo Fisher ScientificBlue (488nm), Red (637nm) Excitation Lasers.
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Flow Cytometry / Cell CountingFlow cytometers are used to count, sort and examine multiple characteristics of cells. Other cell analysis equipment includes image cytometers, cell counters, fluorescence-activated cell sorters (FACS), magnetic-activated cell sorters (MACS), and a range of flow cytometry assay kits. Flow cytometers can reveal information on cell viability, cell proliferation, apoptosis and cell cycle progression, as well as identify cell populations and intracellular or cell-surface molecules. Additionally, some flow cytometers, known as FACS, have an additional sorting function after analysis. Cell counters and image cytometers count live and dead cell populations and can also conduct cell proliferation assays. Find the best flow cytometers, cell counters and cell sorters in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.CardiomyocytesPrecision MedicinePrecision medicine refers to the idea of customized healthcare, where medical decisions and treatments are tailored to the individual patient. Molecular diagnostics, companion diagnostics and Next Generation Sequencing (NGS) play a pivotal role in this approach.