Fast track antibody discovery: A high-throughput method for identifying and isolating rare cells secreting antigen-specific antibodies

In this application note, Sphere Fluidics Ltd., presents a method by which Cyto-Mine^®, utilizing an antigen-specific assay, analyses a heterogeneous B cell or hybridoma population to identify target-specific variants in a high-throughput manner. The Cyto-Mine Single Cell Analysis and Monoclonality Assurance System is the first fully integrated platform designed specifically for biopharma that screens millions of primary B cells or hybridomas for secreted immunoglobulin, identifies and sorts the antigen-specific candidates, and then gently dispenses them into 96- or 384-well microtiter plates with visual proof of cell number, through high-quality imaging.