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Fast and Easy Optimization of Detection Wavelengths with Vanquish Fluorescence Detectors

Fast and Easy Optimization of Detection Wavelengths with Vanquish Fluorescence Detectors

25 Jun 2015

This technical note explains how users can easily optimize the wavelength pair to obtain the best detection using the combination of a Vanquish Fluorescence Detector and Thermo Scientific™ Dionex™ Chromeleon™ Chromatography Data System (CDS) software.

Thermo Scientific™ Vanquish™ Flex Quaternary UHPLC System

Thermo Fisher Scientific

The Thermo Scientific™ Vanquish™ Flex Binary UHPLC system is biocompatible with a state-of-the-art binary high-pressure pump and state-of-the-art binary solvent blending. This ultra high performance liquid chromatography system shares all Vanquish values, such as a design focused on uptime, robustness and reliability. Multiple detection options as well as Thermo Scientific™ Viper™ fingertight connections throughout the system will give you the performance you need with the ease of use you want. Our SmartFlow™ pumping technology with automatic compensation for changing eluent compressibility ensures excellent flow and gradient precision, independent of eluent composition and backpressures. This gives you unrivaled retention time precision and highest data confidence, even for ternary and quaternary gradients, and for more freedom in method development and application switching. Detection capabilities include high sensitivity and linearity Diode Array Detection with LightPipe technology, selective and sensitive Fluorescence Detection and near-universal detection capabilities with the Vanquish Charged Aerosol Detector. Key Features: Integrated modularity design concept to offer uncompromising UHPLC State-of-the-art quaternary solvent blending 1000 bar of pump pressure and flow rates up to 8 mL/min Industry-leading flow and gradient precision Multiple detection options including DAD, FLD and CAD Relevant Applications: Separations of Intact mAbs Analysis of labeled glycans Ternary gradient impurity profiling Efficient, reliable peptide mapping

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UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Fluorescence SpectroscopyFluorometers and spectrofluorometers (also called fluorescence spectrometers) are used to measure the intensity and wavelength of fluorescent light emitted from a sample after excitation by illumination. Spectrofluorometers utilize monochromators to select the desired wavelengths, whereas filter fluorometers employ a set of filters. Spectrofluorometers for measuring steady-state fluorescence and lifetime fluorescence (or time-resolved fluorescence) are available, as well as fluorescence microscopes and microplate readers. Find the best fluorescence spectroscopy products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Software PlatformsSoftware platforms are useful for various stages of laboratory experiments from data collection to data storage and processing. For instance lab software is available for system control, data management, data analysis and qualification / validation.FluorescenceThe emission of fluorescence occurs when a photon of energy is supplied to a fluorescent chemical compound by an external source, causing it to become excited. Fluorescence can be detected and measured for different purposes using microplate readers, fluorescence microscopes, fluorescence scanners, and flow cytometers.ChromatographyChromatography is a powerful technique used to separate and analyze components of mixtures based on their chemical properties. It is widely used in fields like biochemistry, pharmaceuticals, and environmental analysis. By exploiting differences in the interaction of substances with a stationary phase and a mobile phase, chromatography enables precise purification and quantification of compounds. Whether you're working with complex biological samples or industrial chemicals, chromatography is essential for obtaining high-quality results. Browse our peer-reviewed product directory to find the best chromatography systems, compare products, read customer reviews, and get pricing directly from manufacturers.Data AcquisitionData acquisition refers to the collection and organization of information and data sets.
Fast and Easy Optimization of Detection Wavelengths with Vanquish Fluorescence Detectors