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Fast Analysis of Polycyclic Aromatic Hydrocarbons (PAHs) by HPLC-UV

Restek Corp.
13 Nov 2018

Polycyclic aromatic hydrocarbons (PAHs) are a group of nonpolar organic molecules containing two or more benzene rings. PAHs are released from the incomplete combustion of organic fuels, and they generally occur as complex mixtures in our food and environment. Although not all polycyclic aromatic hydrocarbons are known to have detrimental health effects, several PAHs have been demonstrated to be toxic, carcinogenic, or mutagenic. To protect human health, many organizations have mandated exposure limits for PAHs. Air, water, soil, and food samples are commonly tested globally; however, the list of target compounds varies widely around the world. Many HPLC methods recommend a C18 column, but analysis times can be long (~30 minutes) and target analytes are not always completely resolved.

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UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.ChromatographyChromatography is a powerful technique used to separate and analyze components of mixtures based on their chemical properties. It is widely used in fields like biochemistry, pharmaceuticals, and environmental analysis. By exploiting differences in the interaction of substances with a stationary phase and a mobile phase, chromatography enables precise purification and quantification of compounds. Whether you're working with complex biological samples or industrial chemicals, chromatography is essential for obtaining high-quality results. Browse our peer-reviewed product directory to find the best chromatography systems, compare products, read customer reviews, and get pricing directly from manufacturers.PAHsEnvironmental ContaminationContaminants in the environment are harmful chemicals that are present in the air, land and water. These include pesticides, pharmaceuticals, persistent organic pollutants (POPs) and many more.Organic CompoundsOrganic compounds are carbon-based molecules that form the building blocks of life. These include hydrocarbons, lipids, proteins, and nucleic acids. Understanding organic compounds is crucial in chemistry, biochemistry, and pharmacology. Explore organic compound analysis tools in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.UVUltraviolet (UV) light is used in a variety of applications, including disinfection, spectrophotometry, and molecular biology. UV light can damage DNA, making it a powerful tool in sterilization and in studies of mutagenesis and gene repair. Explore UV-related products in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.

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Fast Analysis of Polycyclic Aromatic Hydrocarbons (PAHs) by HPLC-UV