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Fabrication and characterization of nanofluidic devices for DNA optical mapping

ZEISS Research Microscopy Solutions

Fast and flexible nanofluidic prototyping with FIB and SEM

12 Apr 2023

In this application note, ZEISS presents the fabrication and characterization of nanofluidic devices for DNA optical mapping. The devices were produced using FIB-SEM and UV nanoimprint lithography. The focused ion beam (FIB) nanopatterning method enabled the creation of nanochannels of different shapes, cross sections, and depths, including 3D funnel-shaped inlets to optimize DNA flow. The polymer-on-glass stamp created through UV nanoimprint lithography was used to make the actual chip. The local charge compensation technique allowed for high-resolution scanning electron microscopy (SEM) characterization of the devices without any metal coating. The devices have the potential to revolutionize healthcare by enabling fast and affordable DNA screening for personalized medicine.

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NanotechnologyNanotechnology, or nanotech, is an engineering technique using molecular scale functional systems. Applications of nanotechnology include medicine and medical devices, electronics, air and water purification, food science and energy production.Electron MicroscopyElectron microscopes (EM) are used to create high-resolution images of samples at the nanoscale by means of an accelerated beam of electrons as a source of illumination. Types of electron microscope include scanning electron microscopes (SEM), transmission electron microscopes (TEM), scanning transmission electron microscopes (STEM) and cryo-electron microscopes. Focused ion beam (FIB) microscopes are useful for modifying or milling a sample surface with nanometer precision, as well as imaging. Find the best electron microscopes in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.MicroscopyMicroscopy is a technique used to observe small objects in detail, from cells to materials, using light or electron microscopes. It enables researchers to examine structures with high resolution, aiding in fields such as biology, medicine, and materials science. With advanced microscopy techniques, scientists can gain insights into cellular processes, tissue structures, and material properties. Explore the best microscopy solutions in our peer-reviewed product directory, compare products, read customer reviews, and get pricing directly from manufacturers.NanomaterialsNanomaterials such as carbon nanotubes, fullerenes and nanoparticles are a group of materials that measure between 1-1000nm for a single unit. Analysis techniques include AFM, electron microscopy and super resolution microscopy.NanoparticlesNanoparticles are between 1-100nm in size. Nanoparticles can be used for a wide variety of applications including biomedical, catalysis and electronics.

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Fabrication and characterization of nanofluidic devices for DNA optical mapping