Extraction of Opiates From Human Urine Using ISOLUTE® SLE+ 96-Well Plates and Columns
15 Aug 2012This application note describes the extraction of a range of opiates from human urine using ISOLUTE SLE+ supported liquid extraction plates (96-well) and columns with LC-MS/MS analysis.
This method describes the use of ISOLUTE SLE+ supported liquid extraction plates (96-well) and 1 mL sample volume columns to extract a range of opiates including opiate treatment analytes (methadone and its metabolite EDDP)) from human urine. This simplified and efficient extraction method has significant analyte recoveries ranging from 70-102% with LOQs as low as 500 pg/mL.
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ISOLUTE SLE+ Supported Liquid Extraction Products
BiotageISOLUTE® SLE+ plates and columns enable efficient supported liquid extraction of analytes from whole blood, plasma, serum, urine, and oral fluid. The simple load-wait-elute method delivers 99.9% removal of proteins and phospholipids without offline steps, offering superior recovery, reproducibility, and cleaner extracts than liquid-liquid extraction.