ResourceForensics

Extraction of DNA from Hair using forensicGEM® Tissue

11 Aug 2013

Hairs found at crime scenes can be important trace evidence, and in some cases are the one and only link to a suspect. As a source of DNA, hair can prove vital in forensic human identification. Nevertheless, most methods for the extraction of DNA from hair involve a lengthy process that requires extensive manual manipulation. This application note presents a quick method for the extraction of DNA from hair using ZyGEM forensicGEM® Tissue. This method has proven to be a better alternative to other, more time-consuming protocols (for example phenol-chloroform extraction).

forensicGEM™

ZyGEM Corporation Limited

forensicGEM™ has been formulated for high-throughput DNA extraction from crime samples. The forensicGEM™ kits have been specifically designed to be compatible with high-multiplex STR profiling kits and all reagents are rigorously Quality Controlled to test for efficacy and for the absence of human DNA. ZyGEM technology is ideally suited to the large-scale batch processing of reference samples for forensic analysis. Validated by ESR New Zealand, one of the largest forensics laboratories in the world, forensicGEM™ is a rapid, high throughput processing technology for storage card and buccal swab reference samples. ZyGEM also offers a comprehensive range of optimized kits for the preparation of crime scene and CODIS samples. All forensicGEM™ kits are rigorously tested at a leading forensic laboratory for efficacy and purity. The basis of all of the kits is the forensicGEM™ proteinase, but special formulations have been developed for different substrates forensicGEM™ DNA EXTRACTION HAS MANY ADVANTAGES: Closed–tube (protects sample integrity) Hands–off (saves labor costs) One-step (maximizes yields and so increases the chance of obtaining a usable profile) Easily automated (compatible with most low cost, off-the-shelf liquid handling robots) Validated for forensic analysis

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DNA / RNA Extraction and PurificationPurified DNA and RNA are required for numerous downstream molecular biology applications. Consequently, the importance of high-quality DNA/RNA extraction and purification equipment cannot be underestimated. Many purification kits are available and are typically optimized for nucleic acid type and source, including plasmid DNA, genomic DNA, mRNA, RNA and viral nucleic acid purification kits. Automated extraction and purification of nucleic acids can be implemented with magnetic bead separator instruments or high-throughput purification workstations. Find the best DNA/RNA extraction and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Forensic InvestigationForensic investigation is a diverse collection of scientific techniques and methods for the identification and characterization of chemicals relating to crime scene investigations.
Extraction of DNA from Hair using forensicGEM® Tissue