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Extraction of DNA from Cigarette Butts using Proteinase forensicGEM™

ZyGEM Corporation Limited
11 Feb 2013

Cigarette butts are a common trace sample at crime scenes and obtaining DNA profiles from this evidence is an important capability in the forensic science repertoire. In this application note it is shown that the forensicGEM™ Cigarette method is a quick and easy method for processing cigarette butts. While being substantially faster than current organic extraction methods, it is also capable of producing full profiles using AmpFlSTR® Identifiler® – a multiplex fingerprinting system that is particularly sensitive to inhibition.

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forensicGEM™

ZyGEM Corporation Limited

forensicGEM™ has been formulated for high-throughput DNA extraction from crime samples. The forensicGEM™ kits have been specifically designed to be compatible with high-multiplex STR profiling kits and all reagents are rigorously Quality Controlled to test for efficacy and for the absence of human DNA. ZyGEM technology is ideally suited to the large-scale batch processing of reference samples for forensic analysis. Validated by ESR New Zealand, one of the largest forensics laboratories in the world, forensicGEM™ is a rapid, high throughput processing technology for storage card and buccal swab reference samples. ZyGEM also offers a comprehensive range of optimized kits for the preparation of crime scene and CODIS samples. All forensicGEM™ kits are rigorously tested at a leading forensic laboratory for efficacy and purity. The basis of all of the kits is the forensicGEM™ proteinase, but special formulations have been developed for different substrates forensicGEM™ DNA EXTRACTION HAS MANY ADVANTAGES: Closed–tube (protects sample integrity) Hands–off (saves labor costs) One-step (maximizes yields and so increases the chance of obtaining a usable profile) Easily automated (compatible with most low cost, off-the-shelf liquid handling robots) Validated for forensic analysis

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PCR and Thermal CyclingPolymerase chain reaction (PCR) kits and thermal cyclers are used for the in vitro amplification of DNA permitting subsequent analysis and experimental procedures. Explore a range of high-quality polymerase, primers and nucleotides or simplify your workflow with a PCR mastermix. Find reverse transcription PCR (RT-PCR) and cDNA synthesis kits for RNA products and libraries. Quantitatively measure the amplification of DNA with real-time PCR (qPCR) and droplet digital PCR (ddPCR) kits and systems, and discover automated PCR setup solutions to increase throughput. Alternative DNA amplification methods also include recombinase polymerase amplification (RPA) kits. Find the best PCR kits and thermal cyclers and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.DNA / RNA Extraction and PurificationPurified DNA and RNA are required for numerous downstream molecular biology applications. Consequently, the importance of high-quality DNA/RNA extraction and purification equipment cannot be underestimated. Many purification kits are available and are typically optimized for nucleic acid type and source, including plasmid DNA, genomic DNA, mRNA, RNA and viral nucleic acid purification kits. Automated extraction and purification of nucleic acids can be implemented with magnetic bead separator instruments or high-throughput purification workstations. Find the best DNA/RNA extraction and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Evidence Collection and AnalysisEvidence Collection and Analysis is the retrieval and subsequent investigation into criminal evidence obtained from a crime scene. Forensic genetic instruments, kits and reagents enable the analysis of evidence collected  at the crime scene. Reagents and kits for presumptive analysis for body fluids such as blood, saliva and semen in addition to drugs and narcotics test kits are used for CSI. Fingerprinting equipment, forensic light sources, casting kits and equipment for collecting recoverable materials can be found in the directory.Forensic InvestigationForensic investigation is a diverse collection of scientific techniques and methods for the identification and characterization of chemicals relating to crime scene investigations.

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Extraction of DNA from Cigarette Butts using Proteinase forensicGEM™