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Extraction of Corticosteroids from Plasma using Novum Simplified Liquid Extraction (SLE)
12 Feb 2015Corticosteroids are a class of steroid hormones that are widely studied due to their involvement in a variety of physiological processes. The aim of this application note is to introduce a fast and effective sample preparation technique using Novum Simplified Liquid Extraction (SLE) products that results in acceptable recoveries of 7 corticosteroids from plasma.
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Sample PreparationSample preparation can improve the quality and speed of separation techniques. Products to assist sample preparation include filtration equipment, evaporators, membranes and sieves.Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.HormonesPlasma