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Extraction of 25-OH-Vitamin D2 and D3 from Serum by Novum™ Simplified Liquid Extraction and LC/MS/MS Analysis Using a Kinetex® HPLC/UHPLC Column
23 Feb 2015This application note introduces a fast and effective sample preparation technique using Novum Simplified Liquid Extraction (SLE) products that overcomes the challenges observed in a traditional liquid-liquid extraction (LLE). This rapid cleanup is then paired with an LC/MS/MS method using a Kinetex 2.6 μm F5 core-shell HPLC column, resulting in >80 % recoveries.
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UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Sample PreparationSample preparation can improve the quality and speed of separation techniques. Products to assist sample preparation include filtration equipment, evaporators, membranes and sieves.