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Extraction of 22 Pain Management Drugs from Urine using ISOLUTE® SLE+ in 96-Fixed Well Plate Format Prior to LC-MS-MS

Biotage
4 Jul 2012

Read this application note to learn more about the extraction method for 22 different drugs in urine which are typically screened for pain management panels using ISOLUTE SLE+ fixed well plates. The use of schedule I drugs for patient pain management therapy warrants constant monitoring of therapeutic levels in the patient. Screening patient urine samples for the free drugs is complicated by the metabolism process which converts the free drug to the ß-glucuronide form. Patient urine samples can be enzymatically hydrolyzed and extracted to detect the drugs using Supported Liquid Extraction
(ISOLUTE SLE+) which offers an efficient alternative to traditional liquid-liquid extraction (LLE) for bioanalytical sample preparation.

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ISOLUTE SLE+ Supported Liquid Extraction Products

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ISOLUTE® SLE+ plates and columns enable efficient supported liquid extraction of analytes from whole blood, plasma, serum, urine, and oral fluid. The simple load-wait-elute method delivers 99.9% removal of proteins and phospholipids without offline steps, offering superior recovery, reproducibility, and cleaner extracts than liquid-liquid extraction.

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LC-MSLC-MS (liquid chromatography-mass spectrometry) systems and equipment are used for separation and quantitative analysis of complex mixtures, combining liquid chromatography and mass spectrometry. Quantify proteins, contaminants, pesticides or screen for drug metabolites with a high level of sensitivity. LC-MS systems and equipment include reverse phase, normal phase and specialized columns integrated with various MS detectors such as time-of-flight (TOF), quadrupole, orbitrap or ion trap mass analyzers. LC-MS/MS instruments equipped with a qTOF or triple quadrupole analyzer give greater sensitivity and resolving power to your analysis. Find the best LC-MS equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Clinical MicrobiologyMicrobiology is the study of microorganisms including protists, prokaryotes, fungi, and, often, viruses. Microorganisms are a useful research tool as genetic vectors and, in immunology, for antibiotic susceptibility testing, cellular biology and genetics. Microorganisms commonly grow readily in incubators with microbial culture media; this can contain chromogenic supplements to differentiate between cell lines. Estimate your culture’s density of microorganisms with colony counters, or screen and select colonies for desirable clones with automated colony pickers. Additionally, equipment is available to monitor environments for the presence of microbes and identify with microbial identification instruments. Find the best microbiology products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Anti-Doping ScienceAnti-doping science involves the detection of performance-enhancing drugs (PEDs) and banned substances in athletes. This field helps ensure fair competition and athlete safety. Explore anti-doping science tools in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.Pain ManagementUrineThe analysis of urine is important in medicinal, anti-doping, forensic and life sciences applications. The ability to evaluate what someone has ingested through their urine enables fast detection and diagnosis of certain illnesses.

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Extraction of 22 Pain Management Drugs from Urine using ISOLUTE® SLE+ in 96-Fixed Well Plate Format Prior to LC-MS-MS