ResourceLife Sciences
Extraction of 2’-MOE phosphorothioate from tissue using Clarity OTX SPE
14 Sept 2023In this application note, Phenomenex discusses the rising importance of oligonucleotides as a therapeutic option with broad applications. Analyzing oligos in bioanalytical workflows poses unique challenges due to their distinct properties. Achieving relevant limits of quantitation and linearity in pharmacokinetic assays is especially tough due to their low dosing. To overcome these challenges, the note explores a novel single-step solid-phase extraction (SPE) method called Clarity® OTX for extracting a specific splice-switching oligo (SSO) from central nervous system (CNS) tissue.
Links
Tags
OligonucleotidesOligonucleotides are small nucleic acid polymers, usually less than 20 bases in length. Oligonucleotides can be made via enzymatic cleavage or more commonly by chemical synthesis with polymerases. Their use includes FISH, southern blots, microarrays and as primers in PCR. High fidelity synthesis kits and detection systems are available for easy production and detection, respectively.PharmaceuticalsPharmaceuticals are medicinal drugs used in healthcare to diagnose, prevent, cure and treat illnesses. Pharmaceuticals that are excreted after use appear in wastewater and can have detrimental effects on the environment.