ResourceLife Sciences
Expression and Detection of Peptide Tags using pET Express and Purify Kit
10 Aug 2015Peptide tags that are fused to the N- or C-terminus of a protein of interest and specific antibodies generated against these tags provide powerful tools for many applications. Common peptide tags expressed in fusion proteins include his, FLAG, Myc, and HA tags. This application note provides information to help determine the best peptide tag for expression and detection of a target protein.
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Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Tagged ProteinsProtein ExpressionPeptidesPeptides are short chains of amino acids that play key roles in biological processes, including signaling, immune responses, and enzyme regulation. Peptide research is critical in drug discovery, diagnostics, and vaccine development. Browse our peer-reviewed product directory to find the best peptides and peptide-related products, compare options, check reviews, and get pricing directly from manufacturers.