ResourceLife Sciences
Exploring Protein Phosphorylation with RayBio Antibody Arrays and ELISAs
24 May 2017By using antibody arrays and ELISAs, researchers can accomplish high throughput detection of a specific activated protein in multiple samples, or detect phosphorylation signatures through parallel detection of many related phospho-proteins. This application note outline how the RayBio system can be utilized for such applications, as well as a recommended procedure.
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Human/Mouse/Rat Phospho-AKT (S473) and Total Akt ELISA
RayBiotech Inc.RayBio® Human/Mouse/Rat Phospho-Akt (S473) and Total Akt ELISA Kit. This assay semi-quantitatively measures phosphorylated Akt (Ser473) and Total Akt in lysate samples.
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ELISAEnzyme-linked immunosorbent assays (ELISA), also known as enzyme immunoassays (EIA), are used for the detection and quantification of proteins, peptides and antibodies in a sample. ELISA often comes in kit format, with pre-selected antibody pairs to detect specific proteins or biomarkers of diseases such as diabetes and obesity, or cardiovascular and neurological disorders. Detection is made possible with chemiluminescent, fluorescent or colorimetric substrates and detection instruments such as microplate / ELISA readers. Other kits available include: Enzyme-linked immunospot (ELISPOT) assays for the quantification of protein-producing cells and chemiluminescent immunoassays (CLIA) kits. Find the best ELISA kits and products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Microarray AnalysisMicroarrays, also known as biochips, are used for the detection and analysis of multiple genes, proteins, antibodies, or biomarkers on a single microchip. This can reveal information on protein or gene expression, single nucleotide polymorphism (SNP), copy number variation (CNV), epigenetics and patient health in clinical diagnostic tests. Discover a range of microarray scanners and prefabricated antibody, protein, RNA and DNA microarrays for your analysis or consider creating your own custom microarrays with a microarray printer. Find the best microarray products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Antibody ArraysPhosphorylation