ResourceSeparations
Expert Guide to Peptide Mapping
10 Apr 2019In this downloadable Q&A guide, protein separation experts from Phenomenex Inc answer scientists' questions and provide some top tips for peptide mapping.
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UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.LC-MSLC-MS (liquid chromatography-mass spectrometry) systems and equipment are used for separation and quantitative analysis of complex mixtures, combining liquid chromatography and mass spectrometry. Quantify proteins, contaminants, pesticides or screen for drug metabolites with a high level of sensitivity. LC-MS systems and equipment include reverse phase, normal phase and specialized columns integrated with various MS detectors such as time-of-flight (TOF), quadrupole, orbitrap or ion trap mass analyzers. LC-MS/MS instruments equipped with a qTOF or triple quadrupole analyzer give greater sensitivity and resolving power to your analysis. Find the best LC-MS equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.EnzymesEnzymes are macromolecular biological catalysts used across life science, pharmaceutical and clinical research. Various enzymes can be purchased for research applications including ligases, high fidelity polymerases, nuclease, protease, restriction enzymes, tissue dissociation enzymes and enzyme inhibitors. Find the best enzymes in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Protein SeparationPeptide MappingProteomicsProteomics refers to the analysis of proteins through a variety of different experiments in order to understand structure and functionality. Typical techniques include protein purification and mass spectrometry. EnzymesHPLCHigh-performance liquid chromatography (HPLC) is a technique for separating, identifying, and quantifying components in mixtures. It is widely used in chemical, pharmaceutical, and environmental analysis to ensure purity and identify unknown compounds. Explore the best HPLC systems in our peer-reviewed product directory; compare products, read reviews, and get pricing directly from manufacturers.LC-MSLiquid Chromatography-Mass Spectrometry (LCMS) is a powerful analytical technique that combines the separation power of liquid chromatography with the detection capabilities of mass spectrometry. It is widely used for qualitative and quantitative analysis of complex mixtures in pharmaceuticals, proteomics, and environmental studies. Browse our peer-reviewed product directory to find the best LCMS systems, compare products, check reviews, and get pricing directly from manufacturers.