Expansion of Human Mesenchymal Stem Cells Using Corning® HYPER Technology Cell Culture Vessels

7 Aug 2012

This application note describes the use of the Corning® HYPER Technology Platform for the scale-up of hMSCs compared with traditional stacked chamber vessels. Learn how to harvest hMSCs, prepare and scale-up the cultures for both investigative and therapeutic applications...

Corning® CellBIND® Surface HYPERFlask® Cell Culture Vessel

Corning Life Sciences

The High Yield PERformance Flask (HYPERFlask) cell culture vessel has 1720 cm2 available growth area on a polystyrene gas permeable surface. Treated for optimal cell attachment and gamma sterilized. Bar coded and compatible for use on Cell Culture Automation Platforms. Solid cap with liner on a straight neck designed for filling with growth medium. Made with USP Class VI Materials – molded and assembled in a Certified Class 100,000 clean room Sterile – gamma irradiated to sterility assurance level (SAL) 10⁻⁶ Clean Room Packaging – each pack is double bagged. Innovative 10Layer Design – 10 interconnected polystyrene growth surfaces Optimal Growth – Corning CellBIND® Surface treated gas permeable polystyrene for superior cell attachment and growth Increase Cell Yield – 10-fold higher cell yield increases productivity and capacity Time and Space Savings – Reduce processing time and incubator storage space by handling one flask compared to 10 traditional 175 cm2 flasks

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Cell / Tissue CultureCell culture or tissue culture is used to study the biology of cells or tissues and to isolate cellular products in an environment which can be manipulated and well defined. Accurately control your culture environment with bioreactors or culture incubators, bind your cells to a surface or together with an extracellular matrix. Distinguish cell types with differential media or proliferate cells with certain characteristics using selective media. Enrich your media with supplements such as growth factors, sera and vitamins. Find the best cell and tissue culture products, kits and equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell Lines Stem Cells and Primary CellsPrimary cell cultures, established cell lines and stem cells are vital for <i>in vitro</i> and <i>ex vivo</i> experimentation. High-quality cells, optimized for your applications, alongside optimized cell substrates, growth medium and supplements, are critical for experimental success. Explore a range of cells suitable for your applications, including isogenic cell lines, competent cells, induced pluripotent stem cell (iPSC)-derived cell lines, fungal/bacterial/mammalian cell lines, stem cells and cancer cell lines. Find the best cells for your research in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.Bioprocessing / FermentationBioprocessing is the use of biological materials to perform commercial, scientific or medical research processes. Biological materials used include cells, enzymes and organisms. Usually bioprocessing requires a batch or continuous bioreactor such as a fermentor or cell culture system. The advantages of using a reactor include high productivity, easy configuration, adjustable values and automation.hMSCScale-Up
Expansion of Human Mesenchymal Stem Cells Using Corning® HYPER Technology Cell Culture Vessels