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Expanding the GPC/SEC experiment beyond conventional limits using viscometry and light scattering detection

2 Jul 2023

In this application note, Agilent presents the Agilent 1260 Infinity Multi-Detector GPC/SEC System, a powerful tool for polymer characterization. The system utilizes signals from a concentration sensitive detector, such as refractive index (RI) or ultra-violet (UV), along with light scattering and viscometry. These signals enable the determination of the absolute molecular weight, size, and shape of the polymer in solution. The application note demonstrates the value of this technique by analyzing three structurally different materials. It highlights the advantages of using multi-detection GPC/SEC to study a range of polymers.

1260 Infinity II Multidetector GPC/SEC System

Agilent Technologies

With the 1260 Infinity II Multidetector GPC/SEC System, you can select from any combination of detection by refractive index, light scattering, and viscometry. The system delivers accurate, absolute molecular weights and sizes, and can be used to analyze a wide range of polymers, regardless of molecular weight range or solvent. Each detector has independent temperature control to ensure that high levels of precision and reproducibility can easily be achieved from one day to the next.

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1260 Infinity II Multidetector GPC/SEC System

Agilent Technologies

With the 1260 Infinity II Multidetector GPC/SEC System, you can select from any combination of detection by refractive index, light scattering, and viscometry. The system delivers accurate, absolute molecular weights and sizes, and can be used to analyze a wide range of polymers, regardless of molecular weight range or solvent. Each detector has independent temperature control to ensure that high levels of precision and reproducibility can easily be achieved from one day to the next.

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Size Exclusion ChromatographySize exclusion chromatography (SEC) is a liquid chromatographic technique used to separate and identify molecules based on their size, including polymers, proteins, nanoparticles & nucleic acid. SEC is further subdivided into gel filtration chromatography (GFC) and gel permeation chromatography (GPC) when an aqueous or organic mobile phase is used respectively. HPLC-based SEC systems and columns are available for analytical separation alongside SEC spin columns or filters to prep samples for many protocols. Find the best size exclusion chromatography equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Dynamic Light ScatteringDynamic light scattering is used to measure the size distribution of particles in suspension by analyzing the way light scatters off them. It is commonly used in materials science, nanotechnology, and protein characterization. Browse our peer-reviewed product directory to find the best dynamic light scattering systems, compare products, check reviews, and get pricing directly from manufacturers.SECSize exclusion chromatography (SEC) separates molecules based on size, ideal for protein purification, polymer analysis, and biopharma applications. SEC ensures precise characterization of biomolecules for research and industry. Explore peer-reviewed SEC products and compare options for your lab.