ResourceLife Sciences
Excellent assay performance of THUNDER TR-FRET cell-based cytokine assays performed on the PHERAstar FSX
Five THUNDER TR-FRET cytokine assays were tested for compatibility on the PHERAstar FSX
14 Aug 2022Cytokines are important regulators of cell proliferation, differentiation, and immune response1. Since their roles are not completely understood yet, both basic and drug discovery research require accurate cytokine quantification methods. THUNDER™ Biomarker Assays are designed to enable the simple, rapid, sensitive, and robust quantification of biomarkers in cell supernatants. In this application note, BMG LABTECH describes the validation of the PHERAstar FSX multi-mode microplate reader for measuring a panel of five THUNDER™ human cytokine assays.
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PHERAstar® FSX HTS Microplate Reader
BMG LABTECHThe most sensitive microplate reader for high-throughput screening
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Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.CytokinesCytokines are signaling proteins critical for cell communication in the immune system. They regulate inflammation, immune responses, and cell growth. Cytokine research is vital for understanding disease mechanisms and developing therapies. Explore tools for cytokine detection and analysis in our directory.FluorescenceThe emission of fluorescence occurs when a photon of energy is supplied to a fluorescent chemical compound by an external source, causing it to become excited. Fluorescence can be detected and measured for different purposes using microplate readers, fluorescence microscopes, fluorescence scanners, and flow cytometers.Fluorescence Based AssayFluorescence based assays are widely used in life science research and high-throughput screening to measure a broad range of cellular activities.Assay DevelopmentThe process of proving an assay to be sensitive with respect to the target is known as assay development. The assay should be able to characterize novel compounds and measure the potency of these compounds against a validated biological target.