ResourceLife Sciences
Evaluation of X-MAN Standard for EGFR Genotyping Assay
9 May 2012Mutations in the epidermal growth factor receptor (EGFR) gene correlate with the effectiveness of therapy for the treatment of advanced non‐small cell lung cancer (NSCLC). Patients with an EGFR mutation respond more favourably to tyrosine kinase inhibitor therapy, therefore knowledge of their mutation status will aid in developing an effective first‐line course of treatment. In this application note, Horizon’s X-MAN™ genomic DNA Standard was used in the company’s routine EGFR assay to evaluate how efficiently it detects the exon 19 deletion.
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Genome AnalysisGenomics, the study of genomes, includes functional genomics, evolutionary genomics and comparative genomics. There are many genomic technologies such as DNA sequencing of whole genomes, computational biology and bioinformatics. DNA and nucleic acids must be isolated and concentrated from cells for analysis with kits, automated analyzers and software. Other useful technologies for studying genomics include PCR, microarrays and electrophoresis.Clinical GeneticsMolecular Genetics covers the analysis of hereditary genetic disease and chromosomal abnormalities. Genetics can be analysed using DNA, RNA, and protein microarrays, PCR, RT PCR and DNA sequencing. Genetic equipment includes genetic workstations, thermal cyclers, cooling blocks and electrophoresis products. Diagnostic kits are used for DNA / RNA extraction and purification.Next Generation SequencingNext-generation sequencing (NGS), also known as whole-genome sequencing, high-throughput sequencing and massive parallel sequencing, produces and analyses thousands to millions of nucleotide sequences at once. Sequencing systems operate via varying technologies depending on the manufacturer, including sequencing by synthesis, ligation, pyrosequencing, ion semiconductor and single-molecule real-time sequencing. For NGS, library preparation is paramount to successful sequencing. In this section, explore a range of library preparation kits, from targeted, amplicon-based or hybridization-based kits including epigenomic, transcriptomic and genomic workflows to fragmentation kits. Find the best next-generation sequencing products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Genetic StandardsEGFR