Evaluation of the SpectraSlide AP-1 for accurate counting of splenocytes and isolated nuclei

26 Nov 2025

Accurate quantification of tissue-derived samples such as splenocytes and isolated nuclei is essential for a variety of downstream applications, including immunological assays, single-nucleus RNA sequencing (snRNA-seq), and ATAC-seq. Because of their small size, these samples are particularly difficult for image-based cell counters to analyze reliably, requiring both sensitivity and consistency.

This application note, Logos Biosystems evaluates the performance of the SpectraSlide® AP-1 paired with the LUNA-FX7™, focusing on its effectiveness in analyzing tissue-derived samples including splenocytes and isolated nuclei.

SpectraSlide® AP-1

Logos Biosystems

The SpectraSlide® AP-1, designed for the LUNA-FX7™ automated cell counter, is an all-in-one cell counting slide that simplifies the sample prep process - saving time & money. No pipettes and no stains needed! Simply load a sample directly on the slide, insert into the LUNA-FX7™ and press COUNT.

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LUNA-FX7™ Automated Cell Counter

Logos Biosystems

The LUNA-FX7™ is our most powerful cell counter to date, with unmatched cell counting accuracy, new 1, 3 and 8-chamber slide options, state of the art optics, dual fluorescent and brightfield detection, and precision autofocus. The LUNA-FX7™’s new features such as the Bioprocess mode, Quality Control mode, and the CountWire™ (21 CFR Part 11 compliant) software will help companies and facilities meet and surpass quality and regulatory requirements.

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Flow Cytometry / Cell CountingFlow cytometers are used to count, sort and examine multiple characteristics of cells. Other cell analysis equipment includes image cytometers, cell counters, fluorescence-activated cell sorters (FACS), magnetic-activated cell sorters (MACS), and a range of flow cytometry assay kits. Flow cytometers can reveal information on cell viability, cell proliferation, apoptosis and cell cycle progression, as well as identify cell populations and intracellular or cell-surface molecules. Additionally, some flow cytometers, known as FACS, have an additional sorting function after analysis. Cell counters and image cytometers count live and dead cell populations and can also conduct cell proliferation assays. Find the best flow cytometers, cell counters and cell sorters in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.
Evaluation of the SpectraSlide AP-1 for accurate counting of splenocytes and isolated nuclei