ResourceGeneral Lab

Evaluation of Alternatives to Selectrazyme® s-Glucuronidase Reagent for the Analysis of Opiates

6 Jan 2015

Forensic and clinical specimens that screen positive for opiates usually contain opiate glucuronide conjugates in addition to free opiates. Hydrolysis reactions can be performed to cleave β-D-glucuronic acid using an enzyme, acid, or base though consideration must be given to efficiency and the effect it may play on the drug’s chemical structure. Recently, abalone has been presenting itself as a more economical alternative for enzyme hydrolysis. This study investigates the effectiveness of UCT’s Selectrazyme® β-Glucuronidase Reagent (abalone) in comparison to other commercially available abalone derived enzymes.

Selectrazyme™ Beta-glucuronidase

UCT, Inc.

Abalone derived enzyme. Available in both liquid and lyophilized forms. Liquid enzyme activity > 100,000 Units/mL - Lyophilized enzyme activity 1,000,000 - 3,200,000 Units/g

(1)

Links

Tags

Labware and Lab SuppliesLabware and lab supplies include various forms of glassware, plasticware, reagents and personal protective equipment (PPE) and are used across all scientific laboratories, enabling daily tasks to be performed. Explore a range of flasks and beakers, petri dishes, carboys and storage bottles. Labware such as condensers, desiccators and burettes are also available for more specific tasks. Find the best labware and lab supplies in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Solid-Phase ExtractionSolid-phase extraction (SPE) is used for clean-up, extraction and concentration of semi-volatile or non-volatile analytes from complex mixtures including blood, urine and food samples. Multiple formats are available for conducting SPE, including prepacked SPE cartridges, disks and microplates, as well as SPE sorbent powders for manual packing. SPE systems can be used to automate the process and extract multiple samples at once. Solid-phase microextraction (SPME) and supported liquid extraction (SLE) products are also available in the same formats. Find the best SPE, SLE and SPME equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Toxicology / Drugs of Abuse TestingToxicology and Drugs of Abuse Testing is the investigation into toxic and illegal substances found at the scene of a crime or from a suspect and / or victim associated with a scene of a crime. Analyzers, immunoassays and drugs of abuse test kits and presumptive test kits are an essential aspect of toxicology investigation.Forensic ToxicologyForensic toxicology is used to determine cause of death, drug use and contaminations. Toxicological evaluations are commonly used in analytical chemistry, clinical, life sciences and forensics.Opiates
Evaluation of Alternatives to Selectrazyme® s-Glucuronidase Reagent for the Analysis of Opiates