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Ethylene-Bridged [BEH Technology] Hybrids and Their Use in Liquid Chromatography

16 Jan 2019

First Introduced in 1999 in XTerra® columns, Waters patented organic/inorganic Hybrid Particle Technology (HPT) surmounted significant limitations of silica-based reversed-phase packing materials, particularly their hydrolytic instability at high pH. in 2005, second-generation HPT, branded as BEH Technology™, embodied in new ACQUITY UPLC™ BEH Columns and in Waters XBridge™ HPLC columns, marks a new milestone in chromatography.

ACQUITY UPLC BEH Columns

Waters

In 2004, Waters revolutionized liquid chromatography with the introduction of the ACQUITY UPLC System. At the heart of the system was Waters second generation hybrid, the Ethylene Bridged Hybrid (BEH) particle. In 2005, BEH Technology preparative particle sizes (2.5, 3.5, 5 and 10 μm) with the introduction of XBridge between HPLC and UPLC Technology platforms. BEH Technology particles offer: Excellent peak shape and efficiency for basic analytes A rational array of chromatographic selectivity Improvements in chemical stability at mobile phase extremes, particularly at elevated pH

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XBridge Columns

Waters

XBridge Columns include a selection of 10 general-purpose and application-specific sorbents that cover a wide range of particles sizes for analytical and preparative HPLC applications.   XBridge Column users can quickly develop robust methods using mobile phases throughout the entire pH range of 1-12. XBridge OBD™ Preparative Column users, benefit from high pH and temperature stability to provide increased mass loading for basic compounds.   Robust HPLC methods Seamless HPLC to UPLC® method transfer Preparative scaling for product isolation High pH stability Long lifetime and high reliability Maximum method development flexibility High chemical resistance

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UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.ChromatographyChromatography is a powerful technique used to separate and analyze components of mixtures based on their chemical properties. It is widely used in fields like biochemistry, pharmaceuticals, and environmental analysis. By exploiting differences in the interaction of substances with a stationary phase and a mobile phase, chromatography enables precise purification and quantification of compounds. Whether you're working with complex biological samples or industrial chemicals, chromatography is essential for obtaining high-quality results. Browse our peer-reviewed product directory to find the best chromatography systems, compare products, read customer reviews, and get pricing directly from manufacturers.Reversed-Phase LC
Ethylene-Bridged [BEH Technology] Hybrids and Their Use in Liquid Chromatography