Enzymatic Digestion of Polysaccharides Part I: Monitoring Polymer Digestion and Glucose Production in Microplates

14 Oct 2013

The production of ethanol for use as a biofuel from grains or cellulosic material requires the hydrolysis of carbohydrate polymers such as cellulose, xylan and starch to monomeric sugars that are fermentable by cellular organisms such as bacteria and yeast. Here we describe fluorescence detection analysis in microplates of some of the enzymes that catalyze the digestive hydrolysis of polysaccharide polymers into monomeric constituents.

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MicroplatesMicroplates are multi-well plates used to increase the throughput of biological investigations. The number of wells microplates contain can range from 6 to 3243 wells, with the 96-well format being the most commonly used. Microplates can come tailored for a range of applications including cell culture, PCR, filtration, storage, non-binding surface, protein crystallization, as well as pre-coated, deep well and normal Standard microplates. Additionally, options for microplate colors include clear , black, white or black with clear bottom and white with clear bottom for absorbance microplate reader applications. Find the best microplates for your lab in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Biofuel
Enzymatic Digestion of Polysaccharides Part I: Monitoring Polymer Digestion and Glucose Production in Microplates