Enhanced transfection efficiency on protein-coated microplates

19 Feb 2024

In this application note, Greiner Bio-One GmbH details how transfection efficiency in HepG2, CHO, and HEK-293 cells was assessed using CELLCOAT® plates and reporter plasmids. Results showed varying degrees of cytotoxic effects during transfection, with enhanced cell adhesion observed on PDL- and Collagen type I-coated substrates compared to standard cell culture surfaces. Overall, CELLCOAT® plates demonstrated improved suitability for enhancing cell survival and transfection efficiency.

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Cell / Tissue CultureCell culture or tissue culture is used to study the biology of cells or tissues and to isolate cellular products in an environment which can be manipulated and well defined. Accurately control your culture environment with bioreactors or culture incubators, bind your cells to a surface or together with an extracellular matrix. Distinguish cell types with differential media or proliferate cells with certain characteristics using selective media. Enrich your media with supplements such as growth factors, sera and vitamins. Find the best cell and tissue culture products, kits and equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Microplate Readers / DetectorsMicroplate readers are used to automate the detection and analysis of labeled or label-free components in microplates during assays or live-cell monitoring. 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