SelectScience
Join Free
Accelerating Science
ResourceSeparations

Enhanced Detection of Host-Cell Proteins in Biotherapeutic Preparations using Preparative Electrophoresis Followed by LC–Ion Mobility-MS

Waters

Enhanced Detection of Host-Cell Proteins in Biotherapeutic Preparations using Preparative Electrophoresis Followed by LC–Ion Mobility-MS

29 Jun 2015

This application note explains the fractionation of Host-cell proteins (HCPs) away from biotherapeutic protein using an off-line preparative electrophoresis device, allowing for sub-ppm level HCP detection. HCPs are proteins produced by the host cells during recombinant protein drug production and can contaminate the therapeutic drug protein even at very low levels. Detection of HCPs was improved through fractionating both the intact and reduced monoclonal antibody.

Related products

Request Quote for All Products

MALDI SYNAPT G2-Si High Definition Mass Spectrometry

Waters

Redefining Resolution - Resolution in 3 Dimensions. MALDI SYNAPT G2-Si is a uniquely powerful and versatile MS platform. It's the only way to combine multiple dimensions of separation for analysis of complex samples, from imaging to biological research to chemical materials characterization. The MALDI SYNAPT G2-Si gives you high performance, accurate mass MALDI MS/MS, superior post-ionization separation with T-Wave IMS, High Definition Imaging (HDI) MALDI workflows, versatile experimental options, and the ultimate UPLC/MS/MS performance.

(0)

SageELF Sample Fractionator for DNA and Protein

Sage Science

The SageELF is an automated, whole-sample fractionation system for NGS sample prep. SageELF, a whole-sample fractionation tool, is now shipping from Sage Science, developer of the Pippin automated DNA size selection system. The SageELF (short for Electrophoretic Lateral Fractionator) generates 12 contiguous fractions from a whole DNA sample, allowing scientists to generate libraries with multiple insert sizes from the same sample, which has applications in genome sequencing, structural variant detection, and identification of rare splice variants. This sample prep instrument will also be especially useful for preserving precious samples. Users simply load their sample, start the run, and pipette out the fractions later. Unlike in-gel digestion, in which some DNA fragments never leave the gel, the whole DNA sample is electrophoresed into the elution modules.Sage Science targets labor-intensive steps in the sample prep process where automation can offer improved precision and reproducibility. Precise size selection is critical for optimizing sequencing efficiency, improving genome assemblies, and reducing NGS project costs.Features: Make libraries with multiple insert sizes from the same sample Ideal for rare or precious samples Automated for precise, reproducible results Applications: Genome sequencing Structural variant detection Rare splice variant detection

(0)

Links

WatersCompany website

Tags

LC-MSLC-MS (liquid chromatography-mass spectrometry) systems and equipment are used for separation and quantitative analysis of complex mixtures, combining liquid chromatography and mass spectrometry. Quantify proteins, contaminants, pesticides or screen for drug metabolites with a high level of sensitivity. LC-MS systems and equipment include reverse phase, normal phase and specialized columns integrated with various MS detectors such as time-of-flight (TOF), quadrupole, orbitrap or ion trap mass analyzers. LC-MS/MS instruments equipped with a qTOF or triple quadrupole analyzer give greater sensitivity and resolving power to your analysis. Find the best LC-MS equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.AntibodiesAntibodies are used in techniques such as confocal and fluorescence microscopy, flow cytometry, ELISA, ELISPOT, immunohistochemistry, western blotting and immunopreciptation. Select specific antigen reactivity, high specific affinity, low non-specific binding, monoclonal or polyclonal, primary or secondary antibodies and associated conjugates such as an enzyme or dye for visualization.Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.BiotherapeuticsBiotherapeutics are proteins and other compounds (such as nucleic acids) produced by living organisms that have uses as therapeutics or in <i>in vivo</i> diagnostics. The most well-known example of a biotherapeutic product, and the first to be approved for therapeutic use, was recombinant human insulin.ASMSThe American Society for Mass Spectrometry (ASMS) is an organization dedicated to advancing the science and application of mass spectrometry. ASMS supports professionals through events, publications, and research initiatives, promoting innovation in areas like proteomics, metabolomics, and environmental analysis. Drug DevelopmentDrug development refers to the process of bringing a new drug to market.

Related resources