ResourceLife Sciences
Enhance Your Calcium Screens With FLIPR Calcium 6 Assay Kits
Enhance Your Calcium Screens With FLIPR Calcium 6 Assay Kits
10 Sept 2015In this application note, the use of FLIPR Calcium Assay Kits from Molecular Devices is demonstrated in the fluorescent screening of G-protein coupled receptors (GPCRs), ion channels, and other calcium sensitive targets. Using a novel dye formulation to further enhance the calcium flux assay signal window, assay robustness was increased, while providing greater assay protocol flexibility. Certain cells such as Chinese Hamster Ovary (CHO) cell lines have an anion-exchange protein that requires the use of an anion reuptake inhibitor, such as probenecid, to retain commonly used calcium indicators within the cytosol.
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Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.CalciumFluorescenceThe emission of fluorescence occurs when a photon of energy is supplied to a fluorescent chemical compound by an external source, causing it to become excited. Fluorescence can be detected and measured for different purposes using microplate readers, fluorescence microscopes, fluorescence scanners, and flow cytometers.ReceptorsScreeningUsing robotics, data processing and control software, liquid handling devices and sensitive detectors, screening allows a researcher to quickly conduct millions of chemical, genetic or pharmacological tests.DyesCHO