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Endotoxin Removal from Water Buffer, Neutral Sugars, and Certain Biological Solutions

Pall Life Sciences - Laboratory, Food, Beverage

Endotoxin Removal from Water Buffer, Neutral Sugars, and Certain Biological Solutions

5 Feb 2016

This application note from Pall Laboratories describes the removal of endotoxins using the Acrodisc® unit with positively-charged, hydrophilic Mustang® E membrane, which can be used on different solutions. Endotoxin is a complex aggregate of acidic lipopolysaccharides (LPS) and can cause false readings in cell-based assays.

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Acrodisc® WBC Syringe Filter

Pall Life Sciences - Laboratory, Food, Beverage

Pall Life Sciences' Acrodisc WBC premium syringe filter is an innovative filtration device designed specifically for use in the capture and recovery of Leukocytes and the separation of red blood cells. The Acrodisc WBC filter contains Pall's patented Leukosorb media. Leukosorb is a fibrous matrix that uses a multimodal approach of size exclusion and adsorption to capture and recover leukocytes from whole blood samples while allowing Red Blood Cells (RBCs) and platelets to flow through the membrane. With the Acrodisc WBC, the uniquely designed filter does all the difficult capture work, resulting in more efficient white blood cell separation as compared to Buffy Coat density gradient methods. The Acrodisc WBC yields greater cell capture and recovery with less sample compared to larger filter formats and also exudes very low hold up volume as compared to larger white blood cell isolation filters (hold up volume   Applications: Antigenic specificity Antigen modulation studies Cell receptor analysis Lymphocyte recovery for cell therapy Stem cell research applications  

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Acrodisc® PSF 0.2 µm Syringe Filters

Pall Life Sciences - Laboratory, Food, Beverage

Acrodisc PSF syringe filters are available in many membrane types, sizes, and packaging configurations to fit all applications. These are the only syringe filters to receive Caliper Life Sciences Automation Certified guarantee, providing: Smooth operation and worry-free performance in automated applications with smooth filter-to-filter release, consistent turret advancement, exceptional housing strength, and strict outside filter geometry. Best protection for HPLC columns – extend column life as much as 46 times. Maximum throughput with the GxF multi-layer prefilter.

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Sample PreparationSample preparation can improve the quality and speed of separation techniques. Products to assist sample preparation include filtration equipment, evaporators, membranes and sieves.Cell Adhesion AssaysCell adhesion assays are used to quantitate attachment and analyze the molecular mechanisms for extracellular matrix adhesion, cell migration and sensitivity to inhibitors. Find the best cell adhesion assay equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Autoclaves and SterilizersAutoclaves and sterilizers are commonly used to sterilize laboratory equipment, instruments and growth media using constant pressure and temperature. When choosing an autoclave or sterilizer consider medical or laboratory application, size, loading and sterilization method. Sterilization methods include flame (Bunsen burners), heat (steam or dry), chemical and UV.FiltrationFiltration and microfiltration are used to separate solids, particulates or large molecules from fluids (liquid or gases) in order to purify the filtrate. Explore a range of filter formats, including filter paper, plates and membranes for your separation needs. While larger particulates may be separated by gravity filtration, smaller particulates may require additional force for timely separation. Microfiltration equipment includes vacuum filters, as well as positive pressure, crossflow and centrifugal filtration devices. Find the best filtration products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Washing EquipmentWashing equipment is more convenient than ever before with automated systems, integrated and multi-application systems available. For instance microplate washers can be programmable, combined with reagents dispensers and used for applications such as ELISA, multiplex assays and PCR clean up.Assay AssemblyAssay Assembly is technique used in drug discovery to develop assays to test the cytotoxicity, genotoxicity, or other activities of a compound on a cell. Assay assembly requires chip assembly, a delivery system and a detection and analysis method. Beneficial features of assay kits or automated systems include high-throughput, high speed and sensitivity and low signal to noise ratio.Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.Environmental Monitoring and TestingEnvironmental monitoring and testing uses handheld portable analyzers, kits, spectrometers or chromatography systems for air, water, soil, food and other sample testing. Useful features of analyzers such as BOD and COD include portability, easy calibration, automation and sensitivity.  Environmental test kits for pH, water, moisture, etc, should be accurate, sensitive, reliable, fast and easy to use.Particle CharacterizationParticle characterization instruments are used to determine particle size distribution, shape, surface area, zeta potential, density and porosity of particles and materials. Multiple tecchniques are available for determining particle size, shape and count including dynamic light scattering (DLS), laser diffraction, electrozone (Coulter technique), imaging particle analysis and single particle optical sensing. Determine the density of your material with a gas pycnometer or examine its surface area and porosity with gas adsorption analyzers and mercury porosimeters. Find the best particle characterization instruments in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Water PurificationThere are a number of water purification methods available to remove contamination from laboratory water. These systems include demineralization, reverse osmosis, filtration and ultrafiltration. A combination of methods is sometimes necessary to achieve purified water suitable for use in experiments.PurificationPurification techniques are essential for isolating compounds of interest from complex mixtures, such as proteins, nucleic acids, and chemicals. These techniques are critical in biochemistry, drug development, and environmental studies. Explore purification systems in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.AggregationEndotoxinsEnvironmental ContaminationContaminants in the environment are harmful chemicals that are present in the air, land and water. These include pesticides, pharmaceuticals, persistent organic pollutants (POPs) and many more.

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