ResourceSeparations
Enantioselective Resolution and Analysis of Chiral Pesticides in Formulations by UltraPerformance Convergence Chromatography (UPC2) with UV Detection
5 Aug 2015The study of chiral compounds has been a challenge due to the difficulty in resolving the stereoisomers. Convergence chromatography is a complimentary separation technique to liquid chromatography, providing orthogonal selectivity using supercritical CO2 as the primary mobile phase. This application note demonstrates the enantiomeric and/or diastereomeric resolutions of two pesticides, metalaxyl-M (a phenylamide fungicide) and S-metolachlor (acetanilide class of herbicides), and their analyses in aqueous-based pesticide formulations.
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UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Supercritical Fluid ChromatographySupercritical Fluid Chromatography (SFC) is used for the rapid separation and analysis of complex mixtures. SFC systems require injectors, pumps, columns, ovens and detectors. The SFC retention method uses a supercritical fluid, usually CO2. There are a number of different SFC detector types including UV / VIs, ECD, mass spectrometry and fluorescence.Environmental Monitoring and TestingEnvironmental monitoring and testing uses handheld portable analyzers, kits, spectrometers or chromatography systems for air, water, soil, food and other sample testing. Useful features of analyzers such as BOD and COD include portability, easy calibration, automation and sensitivity. Environmental test kits for pH, water, moisture, etc, should be accurate, sensitive, reliable, fast and easy to use.UltraPerformance Convergence ChromatographyConvergence Chromatography expands the capabilities of reversed phase LC and conventional GC techniques. By varying mobile phase strength, pressure, temperature and stationary phase Convergence Chromatography facilitates the separation, detection and quantification of many compounds and samples. The CO<sub>2< / sub> mobile phase used reduces the need for toxic solvents.Chiral CompoundsAgricultureAgriculture research focuses on improving food production, crop management, and sustainability. This includes plant breeding, pest control, and soil health studies. Explore agriculture research tools in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.PesticidesPesticides are used to protect crops from diseases, pests and unwanted plants. They are classed as persistent organic pollutants (POPs) and can be harmful to humans. The Environmental Protection Agency (EPA) currently regulates the use of pesticides in the USA. Chemical Residues in Food