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ELISA workflow guide

Corning Life Sciences
27 Aug 2020

In this application note, Corning provides an overview of the tools you’ll need at each stage of the ELISA workflow, as well as a few tips for choosing the optimal microplate for your particular assay.

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Axygen® Axypet® Pro Pipettors Starter Kit

Corning Life Sciences

Axygen® Axypet® Pro Starter Kit contains 4 variable volume pipettors in the most popular and universal volumes (0.5-10 μL, 2-20 μL, 20-200 μL, and 100-1000 μL), pipet stand for 4 pipettors, and 3 racks with tips. Cat. No. AP-10-P Axygen Axypet Pro Single-channel Pipettor, 0.5 to 10 µL Cat. No. AP-200-P Axygen Axypet Pro Single-channel Pipettor, 20 to 200 µL Cat. No. AP-1000-P Axygen Axypet Pro Single-channel Pipettor, 100 to 1000 µL Cat. No. AP-USD4-P Universal linear stand for four single-channel pipettors Cat. No. MR-10XT-R (10 µL) Axygen Multirack pipet tips Cat. No. MR-200-R (200 µL) Axygen Multirack pipet tips Cat. No. MR-1000XT-R (1000 µL) AxygenSee resource tab below for specifications."

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ELISAEnzyme-linked immunosorbent assays (ELISA), also known as enzyme immunoassays (EIA), are used for the detection and quantification of proteins, peptides and antibodies in a sample. ELISA often comes in kit format, with pre-selected antibody pairs to detect specific proteins or biomarkers of diseases such as diabetes and obesity, or cardiovascular and neurological disorders. Detection is made possible with chemiluminescent, fluorescent or colorimetric substrates and detection instruments such as microplate / ELISA readers. Other kits available include: Enzyme-linked immunospot (ELISPOT) assays for the quantification of protein-producing cells and chemiluminescent immunoassays (CLIA) kits. Find the best ELISA kits and products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.MicroplatesMicroplates are multi-well plates used to increase the throughput of biological investigations. The number of wells microplates contain can range from 6 to 3243 wells, with the 96-well format being the most commonly used. Microplates can come tailored for a range of applications including cell culture, PCR, filtration, storage, non-binding surface, protein crystallization, as well as pre-coated, deep well and normal Standard microplates. Additionally, options for microplate colors include clear , black, white or black with clear bottom and white with clear bottom for absorbance microplate reader applications. Find the best microplates for your lab in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.

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ELISA workflow guide