Efficient uniform isotope labeling of proteins expressed in Baculovirus-infected insect cells using BioExpress 2000

Uniform isotope labeling is a key tool for NMR studies on recombinant proteins and their interaction with ligands of pharmaceutical interest. For this purpose, most recombinant proteins have been expressed in labeled form using E. coli. However, such expression is restricted to proteins of a noncomplex nature. As expression of more complex proteins is routinely done in cell cultures of higher eukaryotes using mammalian or insect cells, the need for isotope-labeling methods for these expression systems is evident.