Efficient Plasmid DNA Isolation from Bacterial Culture with Thermo Scientific KingFisher Flex and KingFisher Pure Plasmid Kit

14 Jan 2015

Efficient plasmid DNA purification from bacterial cultures is key to many molecular-based experiments. This application note describes an automated process for plasmid DNA purification from bacterial cultures. The method integrates the use of the Thermo Scientific™ KingFisher™ Pure Plasmid Kit and the KingFisher Flex platform for efficient purification of plasmids of various sizes. The system was also used to purify plasmids containing recombinant antibody sequences for sequence identification.

Thermo Scientific KingFisher Flex

Thermo Fisher Scientific

The Thermo Scientific KingFisher Flex magnetic particle processor offers great flexibility for high-throughput purification of DNA/RNA, proteins and cells from virtually any source.Using patented magnetic particle separation technology, the KingFisher Flex provides a fast and easy way to get high yields of purified samples with excellent reproducibility and quality.Key Features: High-throughput system processes up to 96 samples in 30 minutes Flexible, open system lets you customize your own purification process Patented magnetic particle processing technology lets you set your protocols and walk away Application Areas: Genetic testing Microbial identification Plant genetics Evolutionary studies / Phylogenetics Protein identification Immunology

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Thermo Scientific KingFisher Pure Kits

Thermo Fisher Scientific

The Thermo Scientific KingFisher Pure Kits are specially designed for use with the KingFisher nucleic acid purification system. These optimized reagent kits help you achieve rapid, high-quality results for a variety of applications – with minimal effort. KingFisher Pure Kits are now available for Cell and Tissue DNA, DNA Blood, DNA Plant, Plasmid, RNA Blood, RNA Plant, RNA Tissue, and Viral NA.Key Features: Developed specifically for use with KingFisher magnetic particle processors Optimized for DNA/RNA purification from a variety of sources Easy-to-use system for pure performance, every time Application Areas: Genetic testing Microbial identification Plant genetics Evolutionary studies / Phylogenetics

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DNA / RNA Extraction and PurificationPurified DNA and RNA are required for numerous downstream molecular biology applications. Consequently, the importance of high-quality DNA/RNA extraction and purification equipment cannot be underestimated. Many purification kits are available and are typically optimized for nucleic acid type and source, including plasmid DNA, genomic DNA, mRNA, RNA and viral nucleic acid purification kits. Automated extraction and purification of nucleic acids can be implemented with magnetic bead separator instruments or high-throughput purification workstations. Find the best DNA/RNA extraction and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Gene Expression and Molecular CloningMolecular cloning is a set of techniques that utilizes vectors to transfer recombinant DNA into host cells and is an essential tool for investigating the expression of genes and proteins in bacterial or mammalian cells. A variety of vectors optimized for gene cloning and expression in a range of host organisms are available, alongside competent cells for genetic replication. Here, you can explore a range of molecular tools, high-quality genomic and cDNA libraries, premade clones, transformation and transfection reagents and mutagenesis or gene expression detection assays and expression arrays. Find the best gene expression and molecular cloning products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Plasmid Purification
Efficient Plasmid DNA Isolation from Bacterial Culture with Thermo Scientific KingFisher Flex and KingFisher Pure Plasmid Kit