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Efficient Method for Scaling-Up Human Mesenchymal Stem Cells using Falcon® Multi-Flasks

Corning Life Sciences
9 Aug 2015

The use of single layer T-flasks can be time consuming, cumbersome and labor intensive for cell culture applications, requiring large numbers of cells. However, ongoing research into the use of multi-layered vessels is required to ensure environmental stability between layers which may affect the yield, homogeneity and potential functional performance of cell populations. In this application note, the success of scaling-up human mesenchymal stem cells (hMSCs) in Falcon® Multi-Flasks is accessed through cell growth and characteristics in comparison to cells grown in control T-175 flasks.

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Falcon® 875cm² Rectangular Straight Neck Cell Culture Multi-Flask, 5-layer with Vented Cap

Corning Life Sciences

Increase productivity by enabling to grow more cells faster and easier. 3- or 5-layer formats provide 525 cm2 and 875 cm2 cell growth surface area, respectively.Even distribution of media across all layers for homogeneous cell growthAbility to mix cells and reagents in the Falcon Multi-Flask saves time and reduces risk of contaminationFlexible design allows to pour or aspirate/recover cells using a pipetConsistent surface treatment for predictable scale-upLot number printed on each flask for traceabilityManufactured in compliance to cGMP standards

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MicroplatesMicroplates are multi-well plates used to increase the throughput of biological investigations. The number of wells microplates contain can range from 6 to 3243 wells, with the 96-well format being the most commonly used. Microplates can come tailored for a range of applications including cell culture, PCR, filtration, storage, non-binding surface, protein crystallization, as well as pre-coated, deep well and normal Standard microplates. Additionally, options for microplate colors include clear , black, white or black with clear bottom and white with clear bottom for absorbance microplate reader applications. Find the best microplates for your lab in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell Lines Stem Cells and Primary CellsPrimary cell cultures, established cell lines and stem cells are vital for <i>in vitro</i> and <i>ex vivo</i> experimentation. High-quality cells, optimized for your applications, alongside optimized cell substrates, growth medium and supplements, are critical for experimental success. Explore a range of cells suitable for your applications, including isogenic cell lines, competent cells, induced pluripotent stem cell (iPSC)-derived cell lines, fungal/bacterial/mammalian cell lines, stem cells and cancer cell lines. Find the best cells for your research in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell ViabilityCell viability assays assess the health of cells and their ability to proliferate. These tests are crucial in drug development, toxicity testing, and cell-based research. Common methods for assessing viability include MTT, live/dead assays, and flow cytometry. Browse our peer-reviewed product directory to find the best tools for cell viability assays, compare products, check reviews, and get pricing directly from manufacturers.Stem Cell ResearchStem cells have the ability to produce identical daughter cells as well as progeny, which commit and differentiate. Stem cell research has exciting prospects of biomedical applications in cellular disease modeling, pharmaceutical screening, and regenerative medicine. Cell GrowthCell growth refers to the process by which cells increase in size and number. It is essential for tissue development, wound healing, and cancer research. Understanding and controlling cell growth is a fundamental part of cell biology and regenerative medicine. Browse our peer-reviewed product directory to find the best tools for studying cell growth, compare products, check reviews, and get pricing directly from manufacturers.Mesenchymal Stem Cells

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Efficient Method for Scaling-Up Human Mesenchymal Stem Cells using Falcon® Multi-Flasks