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Effect of Phosphate on Binding of Proteins to Sartobind STIC® PA

Sartorius Group

Effect of Phosphate on Binding of Proteins to Sartobind STIC® PA

29 Dec 2015

This application note demonstrates the influence of various phosphate concentrations on the binding of proteins and DNA to Sartobind® STIC, which is a salt tolerant anion exchange chromatography membrane. Sartobind® STIC membranes with primary amine (PA) ligands are used during the downstream processing of recombinant proteins for the removal of contaminants such as DNA, host cell proteins, endotoxins and viruses. The anion exchange ligands are coupled to a cellulose membrane which is fitted into a plastic housing for quick handling, making ion exchange purification nearly as easy as filtration. The product is intended for single use and can be applied for contaminant removal from proteins in flowthrough mode (negative chromatography).

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Sartobind STIC® salt-tolerant membrane chromatography

Sartorius Group

Disposable capsules with salt tolerant interaction membranes for polishing in biomanufacturing. The Sartobind STIC® adsorber speeds up the production process by high flow rate and opens new opportunities for the purification of therapeutic proteins and vaccines. Plug and play device Can be used like a filter Enhanced flexibility Reduces validation costs Recommended flow rate of up to 30 bed volumes/min

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Ion ChromatographyIon chromatography, also known as ion exchange chromatography, is a high-performance liquid chromatographic technique used for the separation and identification of ions or polar molecules in a sample, including proteins, nucleotides and amino acids. Equipment includes ion exchange columns, ion exclusion columns, ion chromatography systems, pumps, and detectors. Find the best ion chromatography equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Bioprocessing / FermentationBioprocessing is the use of biological materials to perform commercial, scientific or medical research processes. Biological materials used include cells, enzymes and organisms. Usually bioprocessing requires a batch or continuous bioreactor such as a fermentor or cell culture system. The advantages of using a reactor include high productivity, easy configuration, adjustable values and automation.AutomationAutomation in laboratories and manufacturing processes enhances efficiency, precision, and scalability by reducing the need for manual intervention. It plays a critical role in improving productivity, minimizing human error, and accelerating workflows in fields like diagnostics, drug development, and industrial testing. Automation technologies include robotic systems, automated liquid handlers, and process control systems that streamline complex tasks and ensure consistent, reproducible results. Explore our peer-reviewed product directory to discover the best automation solutions, compare options, read user reviews, and get prices directly from manufacturers.Contamination PreventionContamination prevention aims to control and minimize the risk of contamination during experiments. Biological safety cabinets and sterilization equipment are examples of technologies used to prevent contamination.Protein InteractionsProtein interactions are essential for understanding cellular processes, as proteins work together to carry out biological functions. Studying protein interactions can provide insights into disease mechanisms, drug development, and cell signaling pathways. Explore the best tools for protein interaction research in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.Membrane ProteinsRecombinant Proteins

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