ResourceLife Sciences
Easy automation of metagenomic library preparation with the epMotion® 5073m NGS Solution
19 Aug 2019Metagenomic studies can determine the genetic composition of microbiological samples. These studies usually rely on the use of marker genes, such as 16S rDNA, for the phylogenic classification. Which specific marker gene and region will be examined and how deeply sequenced will depend on the aim of the study. Nevertheless, the basic approach of library generation is common to these experimental designs. It is important to have a robust workflow to correctly analyze the samples. In this application note we demonstrate such a workflow, which may be customized and scaled to specific customer needs.
Links
Tags
Next Generation SequencingNext-generation sequencing (NGS), also known as whole-genome sequencing, high-throughput sequencing and massive parallel sequencing, produces and analyses thousands to millions of nucleotide sequences at once. Sequencing systems operate via varying technologies depending on the manufacturer, including sequencing by synthesis, ligation, pyrosequencing, ion semiconductor and single-molecule real-time sequencing. For NGS, library preparation is paramount to successful sequencing. In this section, explore a range of library preparation kits, from targeted, amplicon-based or hybridization-based kits including epigenomic, transcriptomic and genomic workflows to fragmentation kits. Find the best next-generation sequencing products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Library PreparationLibrary preparation is a critical step in sequencing workflows, where DNA or RNA samples are converted into libraries for high-throughput, next generation sequencing. This step ensures accurate results and minimizes biases. Explore library preparation kits in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.Metagenomics