ResourceLife Sciences
Dynamic evaluation of transgene expression and transfection performance using real-time live cell analysis system
20 Jan 2026This application note demonstrates how the Spica M1 real-time live-cell analysis system enables dynamic, multi-parameter evaluation of transgene expression and transfection performance. Using GFP reporter assays in 293T cells, the study highlights continuous monitoring of expression kinetics, expressivity, penetrance, and cell viability, revealing critical insights that traditional endpoint assays miss. See how the Spica M1 supports data-driven optimization of transfection conditions while preserving cell health, accelerating gene expression research, and cell line development.
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Live Cell ImagingLive cell imaging is the study of living cells using microscopes and high-content imaging systems. This technique provides in-depth insight into fast and complex biological processes, by allowing dynamic imaging of living cells instead of acquiring an individual image at a single point in time.Real-Time ImagingCell ImagingCell imaging can be achieved using a number of techniques including confocal microscopy, transmission electron microscopy, atomic force microscopy, and light sheet microscopy.