ResourceSpectroscopy
Dye‐Protein Binding Monitored in a Microliter Volume Using Time-Resolved Fluorescence
11 Dec 2014The DeltaFlex system, with the Microsense cuvette, can perform time‐resolved fluorescence to obtain kinetic information on processes occurring on the millisecond time scale in microliter volumes. In this application note, the interaction of a dye binding to a protein was monitored. This was achieved by the rapid and efficient data-acquisition enabled by the 100 MHz excitation rate, coupled with the low dead time of the timing electronics. The use of time‐resolved fluorescence enables a fuller picture to be obtained, by providing a further fluorescence parameter (the fluorescence lifetime) with the advantage that this is also independent of concentration.
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Fluorescence SpectroscopyFluorometers and spectrofluorometers (also called fluorescence spectrometers) are used to measure the intensity and wavelength of fluorescent light emitted from a sample after excitation by illumination. Spectrofluorometers utilize monochromators to select the desired wavelengths, whereas filter fluorometers employ a set of filters. Spectrofluorometers for measuring steady-state fluorescence and lifetime fluorescence (or time-resolved fluorescence) are available, as well as fluorescence microscopes and microplate readers. Find the best fluorescence spectroscopy products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Protein BiologyThe analysis of protein expression, identity and function is vital for many areas of life science research and drug discovery. Some of the most commonly used techniques in protein analysis include Western blotting, electrophoresis and mass spectrometry.