ResourceSpectroscopy

DNA Quantitation in Cuvette and TrayCell using the Thermo Scientific Multiskan GO Spectrophotometer

29 Nov 2010

This application note describes how to perform photometric DNA quantitation and qualification measurements with the Thermo Scientific Multiskan GO microplate and cuvette spectrophotometer in various cuvette formats. By choosing the right cuvette(s), it is possible to easily cover a wide concentration range in DNA quantitation measurements.

Thermo Scientific Multiskan GO

Thermo Fisher Scientific

The Thermo Scientific Multiskan GO UV/Vis microplate spectrophotometer offers free wavelength selection for both 96- and 384-well plates and various types of cuvettes. It features a broad wavelength range with UV area and pathlength correction, as well as fast spectral scanning; making it ideal for virtually any photometric research application, including DNA, RNA and protein analysis. The Multiskan GO can be controlled as a stand-alone with straight-forward internal software for quick and simple measurements. And even the most demanding applications can easily be set up using the intuitive Thermo Scientific SkanIt software for PC control. Both the internal and PC software are available in multiple languages including English, French, German, Spanish, Portuguese, Russian, Chinese and Japanese.Key Features: Performs endpoint, kinetic and spectral scanning assays for 96- and 384-well plates, and cuvettes Compatible with Thermo ScientificTM μDropTM Plate – a quick and easy tool for DNA and RNA assays using sample volumes down to 2 μL Fast plate measurements - full spectrum of a sample in less than 10 seconds High quality data guaranteed by extensive self-diagnostics Automation compatible Application Areas: DNA and RNA quantification and purity Protein assays Immunoassays (ELISA) Cell proliferation and cytotoxicity assays Enzyme assays Reporter gene assays

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UV-Visible SpectroscopyUltraviolet-visible (UV-Vis) spectrophotometers are used to measure the interaction of UV and visible light with a sample, including transmission, reflectance & absorbance. The two major instrument classes are single-beam or double-beam spectrophotometers. More specialized equipment includes colorimeters, spectroradiometers and refractometers. Portable and microvolume spectrophotometers are also available. For the modular spectroscopy lab, explore a range of light sources for combination with a spectrograph/spectrometer and optics. Find the best UV-Vis spectroscopy products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Genome AnalysisGenomics, the study of genomes, includes functional genomics, evolutionary genomics and comparative genomics. There are many genomic technologies such as DNA sequencing of whole genomes, computational biology and bioinformatics. DNA and nucleic acids must be isolated and concentrated from cells for analysis with kits, automated analyzers and software. Other useful technologies for studying genomics include PCR, microarrays and electrophoresis.DNA SequencingDNA sequencing, such as sanger sequencing, is a biological technique that determines the precise order of nucleotide bases in a fragment or template of DNA. DNA sequencers and genetic analyzers are based on capillary electrophoresis, where labeled DNA fragments are electrophoretically separated by size as they migrate through a polymer. Find the best DNA sequencing products, including DNA sequencing kits, genomic libraries and genetic identity kits in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.
DNA Quantitation in Cuvette and TrayCell using the Thermo Scientific Multiskan GO Spectrophotometer