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DNA human identification from bone fragments using the Precellys 24 Touch

Bertin Technologies
7 Apr 2022

Bone fragment is the most common sample used for DNA human identification from carbonized bodies or corpses, where other organic material is lacking. The scarce DNA in this type of evidence can often prevent traditional methodologies for DNA extraction and purification, such as phenol-chloroform with Proteinase K digestion, from generating a conclusive result.

In this application note, discover how a new methodology based on the Precellys® 24 Touch can provide improved yield and recovery of genetic loci from bone samples in forensic routines.

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Precellys® 24 Touch

Bertin Technologies

Precellys ® 24 Touch is the ideal lab homogenizer to prepare any kind of biological sample, from the softest to the hardest. It can treat up to 24 samples at a time and has been validated on more than 1,500 different samples. The intuitive touchscreen interface of Precellys ® 24 Touch makes it really easy to use. Thanks to its exclusive vacuum-based tube locking system and its specific design, Precellys ® 24 Touch is compliant with the harshest safety requirements, such as BSL3.

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DNA / RNA Extraction and PurificationPurified DNA and RNA are required for numerous downstream molecular biology applications. Consequently, the importance of high-quality DNA/RNA extraction and purification equipment cannot be underestimated. Many purification kits are available and are typically optimized for nucleic acid type and source, including plasmid DNA, genomic DNA, mRNA, RNA and viral nucleic acid purification kits. Automated extraction and purification of nucleic acids can be implemented with magnetic bead separator instruments or high-throughput purification workstations. Find the best DNA/RNA extraction and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.DNA / RNA QuantificationDetection and quantification of nucleic acids is important in molecular biology, cloning, expression, forensics and clinical diagnostics. Nucleic acids can be detected by labeling with colorimetric, fluorescent or radio labels and using in situ hybridization kits to identify specific sequences. Multiple nucleic acids can be detected and quantified at once using RNA / DNA detection beads or RNA / DNA microarrays. Find the best DNA / RNA Quantification products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.DNA ExtractionDNA extraction is a critical step in many biological studies, including genomics, diagnostics, and forensic analysis. The process involves isolating DNA from cells or tissues, and the quality and purity of the extracted DNA can impact downstream applications. Explore DNA extraction products in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.Forensic InvestigationForensic investigation is a diverse collection of scientific techniques and methods for the identification and characterization of chemicals relating to crime scene investigations.

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DNA human identification from bone fragments using the Precellys 24 Touch