ResourceLife Sciences
DNA-Free Reagents and Materials for Molecular Analysis of Bacterial and Fungal Pathogens
8 Nov 2018Microbial DNA is present at very low loads in clinical specimens. Molecular detection by amplification assays generally is a challenge because of a potentially multiple input of contaminating DNA from exogenous sources. Besides air-borne, handling and cross contamination, materials and reagents used in the molecular laboratory can contain microbial DNA which is a long underestimated potential source of false positive results. In this contribution decontamination procedures of material and reagents and the selection of certified microbial DNA-free components for sample collection, DNA extraction and PCR amplification are discussed with respect to the aim of building up a reliable molecular system for the analysis of bacterial and fungal organisms at the limit of detection.
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DNA / RNA Extraction and PurificationPurified DNA and RNA are required for numerous downstream molecular biology applications. Consequently, the importance of high-quality DNA/RNA extraction and purification equipment cannot be underestimated. Many purification kits are available and are typically optimized for nucleic acid type and source, including plasmid DNA, genomic DNA, mRNA, RNA and viral nucleic acid purification kits. Automated extraction and purification of nucleic acids can be implemented with magnetic bead separator instruments or high-throughput purification workstations. Find the best DNA/RNA extraction and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Next Generation SequencingNext-generation sequencing (NGS), also known as whole-genome sequencing, high-throughput sequencing and massive parallel sequencing, produces and analyses thousands to millions of nucleotide sequences at once. Sequencing systems operate via varying technologies depending on the manufacturer, including sequencing by synthesis, ligation, pyrosequencing, ion semiconductor and single-molecule real-time sequencing. For NGS, library preparation is paramount to successful sequencing. In this section, explore a range of library preparation kits, from targeted, amplicon-based or hybridization-based kits including epigenomic, transcriptomic and genomic workflows to fragmentation kits. Find the best next-generation sequencing products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.DNA ExtractionDNA extraction is a critical step in many biological studies, including genomics, diagnostics, and forensic analysis. The process involves isolating DNA from cells or tissues, and the quality and purity of the extracted DNA can impact downstream applications. Explore DNA extraction products in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.DNADeoxyribonucleic Acid (DNA) is the main component of chromosomes and the carrier of genetic information of living organisms. Find out here about PCR, NGS, ChIP-Seq, gel imaging, and many other techniques which can be used for the analysis of DNA.Pathogen DetectionPathogen detection is the process of identifying harmful microorganisms, including bacteria, viruses, and fungi, that can cause diseases. Accurate pathogen detection is critical for food safety, public health, and clinical diagnostics. Explore pathogen detection products in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.MicrobiomeMicrobiome research focuses on the complex communities of microorganisms living in and on humans, animals, plants, and environments. It plays a crucial role in health, disease, and ecological balance. Explore tools and resources for microbiome analysis, including sequencing platforms and bioinformatics software.