SelectScience
Join Free
Accelerating Science
ResourceLife Sciences

DNA and RNA extraction from FFPE tissue

SelectScience

Take your DNA and RNA extraction from FFPE tissue to the next level

25 Sept 2022

Formalin-fixed paraffin-embedded (FFPE) tissue preservation is a method that was first developed 125 years ago to maintain tissue morphology. With the advent of DNA sequencing and other modern molecular methods in the past few decades, there has been great demand to extract DNA and RNA from FFPE tissue. However, FFPE is an imperfect sample type for such analyses because the nucleic acids are often highly degraded and cross-linked to proteins, as a result of the fixation process. Nevertheless, the dominance of FFPE samples in archives and its use in prospective studies means FFPE is likely to be widely used for years to come.

This application eBook provides an expert guide on how to improve and optimize DNA and RNA extraction from FFPE tissue, outlines isotachophoresis, explores new methods to enhance nucleic acid extraction and purification, and provides valuable information on how to effectively identify novel biomarkers from FFPE samples.

This eBook will also cover:

  • New technologies and solutions designed to enhance nucleic acid extraction and purification
  • Methods to generate higher yields with simple and automated workflows

Related products

Request Quote for All Products

Ionic® Purification System

Purigen Biosystems

The Ionic® Purification System is a benchtop platform that provides automated extraction of DNA and RNA from biological samples. Using cutting-edge technology based on isotachophoresis, the Ionic system separates DNA and RNA freely in solution, without binding to or stripping nucleic acids from physical surfaces. The workflow is simple and efficient, while producing higher yields with higher purity, consistently and reliably.

(0)

Links

SelectScienceCompany website

Tags

Cell / Tissue CultureCell culture or tissue culture is used to study the biology of cells or tissues and to isolate cellular products in an environment which can be manipulated and well defined. Accurately control your culture environment with bioreactors or culture incubators, bind your cells to a surface or together with an extracellular matrix. Distinguish cell types with differential media or proliferate cells with certain characteristics using selective media. Enrich your media with supplements such as growth factors, sera and vitamins. Find the best cell and tissue culture products, kits and equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.DNA / RNA Extraction and PurificationPurified DNA and RNA are required for numerous downstream molecular biology applications. Consequently, the importance of high-quality DNA/RNA extraction and purification equipment cannot be underestimated. Many purification kits are available and are typically optimized for nucleic acid type and source, including plasmid DNA, genomic DNA, mRNA, RNA and viral nucleic acid purification kits. Automated extraction and purification of nucleic acids can be implemented with magnetic bead separator instruments or high-throughput purification workstations. Find the best DNA/RNA extraction and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Tissue ProcessingTissue processing involves preparing biological tissue samples for microscopic analysis, including fixation, embedding, and sectioning. This process is essential for histopathology and diagnostic applications. Explore tissue processing products in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.DNA ExtractionDNA extraction is a critical step in many biological studies, including genomics, diagnostics, and forensic analysis. The process involves isolating DNA from cells or tissues, and the quality and purity of the extracted DNA can impact downstream applications. Explore DNA extraction products in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.DNADeoxyribonucleic Acid (DNA) is the main component of chromosomes and the carrier of genetic information of living organisms. Find out here about PCR, NGS, ChIP-Seq, gel imaging, and many other techniques which can be used for the analysis of DNA.RNARNA is a nucleic acid that plays a key role in gene expression and protein synthesis. It serves as a messenger between DNA and ribosomes, carrying genetic instructions to produce proteins. Advances in RNA-based therapies, such as mRNA vaccines and gene editing, have revolutionized treatment strategies for genetic disorders and infectious diseases. Explore RNA research tools and therapies in our peer-reviewed product directory; compare products, check customer reviews, and get pricing directly from manufacturers.FFPEFormalin-fixed paraffin-embedded (FFPE) tissues are commonly used in medical research, particularly in histology and cancer studies. The preservation process allows long-term storage of tissue samples for later analysis. Explore FFPE research products in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.DNA QuantificationDNA quantification is the process of measuring the amount of DNA in a sample. It is vital in genomics, molecular biology, and biotechnology, helping to ensure accurate experiments and data analysis. Explore DNA quantification tools in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.

Related resources