ResourceLife Sciences
Direct PCR from Plant Material is a Challenging Application as a Result of the Diversity of Plant Tissue Types and the Potent PCR Inhibitors Contained within the Tissue
19 Sept 2014Success rates with direct PCR are typically low due to the high concentrations of inhibitors present in even small amounts of plant material, and the precise control of sample size required. This Application Note demonstrates a systematic approach for reaction optimization with the KAPA3G Plant PCR Kit, as well as a robust method for successful crude sample PCR.
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PCR and Thermal CyclingPolymerase chain reaction (PCR) kits and thermal cyclers are used for the in vitro amplification of DNA permitting subsequent analysis and experimental procedures. Explore a range of high-quality polymerase, primers and nucleotides or simplify your workflow with a PCR mastermix. Find reverse transcription PCR (RT-PCR) and cDNA synthesis kits for RNA products and libraries. Quantitatively measure the amplification of DNA with real-time PCR (qPCR) and droplet digital PCR (ddPCR) kits and systems, and discover automated PCR setup solutions to increase throughput. Alternative DNA amplification methods also include recombinase polymerase amplification (RPA) kits. Find the best PCR kits and thermal cyclers and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.PCR PurificationPCR AutomationPCR automation describes the process of using a thermocycler to complete the polymerase chain reaction (PCR) process quickly and efficiently.