Direct PCR from Blood Preserved on Whatman FTA and 903 Cards using Thermo Scientific Phusion Blood Direct PCR Kit

6 Feb 2014

Paper cards are a popular method of storing blood due to their ease of use and long term stability at room temperature. However amplification of DNA directly from storage cards samples is particularly challenging due to PCR inhibitors present. With conventional DNA polymerases, a thorough washing protocol is required. This study demonstrates that Phusion Blood Direct PCR Kit allows amplification of DNA directly from blood stored on cards with no or very little pretreatment.

Phusion Blood Direct PCR Kit

Thermo Fisher Scientific

Thermo Scientific Phusion Blood Direct PCR Kit is designed for amplification of DNA from whole blood. It eliminates the need for a separate DNA purification step prior to PCR. The modified Phusion Hot Start II High-Fidelity DNA Polymerase is resistant to PCR inhibitors present in blood and retains its activity even with samples containing 40% whole blood. This extremely accurate proofreading DNA polymerase makes the Phusion Blood Direct PCR Kit an ideal choice even for demanding applications, such as cloning. Phusion Blood Direct PCR Kit also allows analysis of blood samples with different anticoagulants, storage conditions and from various species.Phusion Blood Direct PCR Kit Features: No need for time-consuming and expensive DNA purification steps. Simple protocol requires minimal hands-on time. Extremely short PCR protocol times. Modified Phusion Hot Start II High-Fidelity DNA Polymerase delivers abundant yields of specific product. Phusion Blood Direct PCR Kit Includes: Phusion Blood II DNA Polymerase. 2X Phusion Blood PCR Buffer (includes dNTPs and MgCl2). 50 mM EDTA. 50 mM MgCl2. DMSO. Universal Control Primer Mix.

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PCR and Thermal CyclingPolymerase chain reaction (PCR) kits and thermal cyclers are used for the in vitro amplification of DNA permitting subsequent analysis and experimental procedures. Explore a range of high-quality polymerase, primers and nucleotides or simplify your workflow with a PCR mastermix. Find reverse transcription PCR (RT-PCR) and cDNA synthesis kits for RNA products and libraries. Quantitatively measure the amplification of DNA with real-time PCR (qPCR) and droplet digital PCR (ddPCR) kits and systems, and discover automated PCR setup solutions to increase throughput. Alternative DNA amplification methods also include recombinase polymerase amplification (RPA) kits. Find the best PCR kits and thermal cyclers and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Clinical ChemistryBiochemistry (or clinical chemistry) involves the analysis of bodily fluids using chemical tests. Techniques used include HPLC, chromatography, spectroscopy, mass spectrometry, immunochemical, electrophoresis, turbidometric / spectrophotometric assay, MRI and ISE analysis. Tests are often carried out on plasma or serum but urine (urinalysis) and fecal specimens are also processed.qPCRQuantitative PCR (qPCR) is a molecular biology technique used to amplify and measure the amount of a specific DNA or RNA target. It plays a crucial role in gene expression analysis, pathogen detection, and quantifying DNA or RNA in various applications. Real-time PCR provides precise, quantitative results and is widely used in research, diagnostics, and clinical studies. Browse our peer-reviewed product directory to find the best qPCR solutions; compare products, check reviews, and get pricing directly from manufacturers.DNA AmplificationDNA Amplification is a technique used to amplify a single or multiple copies of DNA or mRNA by orders of magnitude. The most common method employed is PCR, but other options exist that eliminate the need for thermocycling.FTA Card
Direct PCR from Blood Preserved on Whatman FTA and 903 Cards using Thermo Scientific Phusion Blood Direct PCR Kit