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Direct Analysis of Red Wine Using Ultra-Fast Chromatography and High Resolution Mass Spectrometry

7 Apr 2014

This application note demonstrates the feasibility of analyzing complex mixtures without any prior sample preparation by making use of the high resolving power of both U-HPLC and the Orbitrap™ mass analyzer detector.

Thermo Scientific LTQ Orbitrap XL

Thermo Fisher Scientific

Thermo Scientific LTQ Orbitrap XL - The Most Confident Protein Identification The Thermo Scientific LTQ Orbitrap XL hybrid FTMS (Fourier Transform Mass Spectrometer) supports a wide range of applications from routine compound identification to the most challenging analysis of low level components in complex mixtures. The LTQ Orbitrap XL is based on the fast and highly sensitive Thermo Scientific LTQ XL linear ion trap and the patented Orbitrap* technology. The LTQ Orbitrap XL features a new HCD collision cell for ultimate flexibility in fragmentation experiments for advanced proteomics and small molecule research. Key features of the Thermo Orbitrap XL include: Outstanding Mass Accuracy, Resolution, Dynamic Range and Sensitivity • Featuring the new HCD collision cell for ultimate flexibility in fragmentation experiments for advanced proteomics and small molecule research • Superior mass accuracy for lower false positive rates (FPRs) • Parallel acquisition mode for optimized productivity From Protein ID to Biomarker Discovery with the Thermo LTQ Orbitrap• Excellent sensitivity and fragmentation efficiency • Reliable identification and quantitation in one experiment High quality data from the LTQ Orbitrap XL for reliable de novo sequencing • Superior mass accuracy and high resolution leading to confident de novo sequencing results • Multiple collision modes (CID and HCD) for comprehensive structural information

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UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Mass SpectrometryMass spectrometry (MS) is a powerful analytical technique used to identify and quantify molecules based on the mass-to-charge ratio of gas-phase ions. It provides detailed information about the structure, composition, and properties of compounds and is widely used across fields such as environmental monitoring, materials science, drug discovery and development, food and beverage testing, and wider chemical research. Key MS techniques include tandem mass spectrometry (MS/MS), liquid chromatography–mass spectrometry (LS-MS) and inductively coupled plasma (ICP-MS). Choosing from these wide range of techniques and technologies can be a daunting task, so keep up to date with scientific applications, performance expectations, and customer reviews here all in one place. Visit our product directory to receive quotes direct from the manufacturer. WineWineAlcoholic BeveragesThe analysis of alcoholic beverages involves testing their composition, quality, and safety, including alcohol content, flavor compounds, and impurities. This is essential for quality control and regulatory compliance. Explore tools for alcoholic beverage testing in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.Beverage AnalysisBeverage analysis involves testing and evaluating the composition, quality, and safety of drinks, from alcoholic beverages to soft drinks. Techniques like chromatography and spectroscopy are commonly used to analyze flavors, nutrients, contaminants, and additives. Browse our peer-reviewed product directory to find the best beverage analysis tools, compare products, check reviews, and get pricing directly from manufacturers.
Direct Analysis of Red Wine Using Ultra-Fast Chromatography and High Resolution Mass Spectrometry