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Development of Recombinant Protein Overproducer Cell Lines with Lentiviral Expression Vectors

Cellecta
6 Dec 2018

Custom overproducer cell line services are offered using the developed protocols, for the development of a wide range of cell lines overproducing recombinant proteins. Using Cellecta’s lentiviral system, a construct containing the gene of interest can be packaged into VSV-g pseudotyped viral particles and delivered into a wide range of mammalian cells. With an average development time of 6 weeks, these cell lines dramatically improve the efficiency of process development and can be used for preparative and industrial-scale production of proteins.

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Ready-to-Use Lentiviral Packaging Plasmid Mix

Cellecta

Cellecta’s Ready-to-Use Lentiviral Packaging Plasmid Mix allows the production of pseudoviral particles when mixed with second- or third-generation lentiviral vectors. The Packaging Plasmid Mix is a mixture of the human immunodeficiency virus (HIV) lentiviral packaging plasmid psPAX2 and the pMD2.G plasmid containing VSV-G. These plasmids provide all the necessary structural, regulatory, and replication proteins required to produce pseudotyped packaged lentiviral expression constructs when co-transfected with an HIV-based lentiviral expression construct (e.g. Cellecta pRSI shRNA expression constructs) into a producer 293T cell line (e.g. ATCC, Cat.# CRL-11268™). Pseudotyped lentiviral particles with VSV-G envelope protein can infect a variety of mammalian or non-mammalian, dividing or non-dividing cells. The kit contains 250 µg of lentiviral packaging plasmid mix that will be sufficient for 25 transfections in 10-cm culture plates. Cellecta’s packaging mix contains tested anion-exchange purified packaging plasmids pre-mixed at the proper ratio for optimal production of packaged VSV-g pseudotyped lentiviral particles. This reagent is used in conjunction with HEK 293T cells. The Ready-to-Use Lentiviral Packaging Plasmid Mix is compatible with most commercially-available second- and third-generation lentiviral vectors.

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ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Protein ExpressionProtein expression is the utilization of cell machinery for the synthesis of proteins and has become a critical tool in biotherapeutic, genomic, and proteomic research. Produce recombinant proteins with expression vectors in combination with a host cell suitable for high-level protein expression. For production of toxic proteins, consider cell-free expression vectors. Create and monitor post-translational modifications with protein modification kits. Find the best protein expression products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Process DevelopmentProcess development aims to optimize the performance of manufacturing systems. This involves the assessment of quality and efficiency of both processes and products. This is particularly important in the scale-up of chemical processes.Recombinant ProteinsVirusesViruses are microscopic pathogens that require a host cell to replicate. Understanding their structure, replication cycle, and impact on the immune system is crucial in developing effective treatments, vaccines, and diagnostic methods. Research continues to focus on emerging viral diseases and antiviral drug discovery. Explore solutions for virus research, detection, and treatment in our peer-reviewed product directory; compare products, check customer reviews, and get pricing directly from manufacturers.Cell CultureCell culture involves growing cells under controlled conditions, typically outside of their natural environment, to study cellular behavior, drug response, and disease models. Cell culture is crucial in biotechnology and biomedical research. Explore cell culture products in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.

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Development of Recombinant Protein Overproducer Cell Lines with Lentiviral Expression Vectors