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Development of High Capacity Magnetic Protein A Beads and Protein G Beads for Rapid Antibody Purification, Characterization and Drug Conjugation

Promega Corp.
13 Jul 2015

MagneTM Protein A Beads and MagneTM Protein G Beads purify polyclonal and monoclonal antibodies from serum, ascites fluid and cell media. High capacity magnetic Protein G and Protein A beads can be used to conjugate antibodies with fluorescent dyes and small molecules for ADC applications, with no need of purified antibodies, dialysis or buffer exchange or parallel labelling of samples. This scientific poster describes the use of MagneTM High Capacity Protein A and Protein G beads in rapid antibody purification, characterization and drug conjugation.

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Magne™ Protein A and Magne™ Protein G Beads

Promega Corp.

Magne™ Protein G and Magne™ Protein A Beads are magnetic affinity beads with high specificity and high capacity for purification of immunoglobulins from cell culture media, ascites and serum samples.   These paramagnetic beads are composed of iron encapsulated in macroporous cellulose with low nonspecific binding. The magnetic beads use a novel attachment chemistry to immobilize recombinant Protein G or Protein A protein molecules in the same orientation on the surface of the bead. The oriented attachment is known to improve the functionality of immobilized proteins. These beads offer a convenient method for achieving high purity and high recovery of monoclonal and polyclonal antibodies from a variety of biological samples. The superb magnetic properties of Magne™ Protein G and Magne™ Protein A Beads allow rapid and efficient capture of antibodies either with manually processed samples or in a high-throughput manner using the Promega ReliaPrep™ LV 32 HSM Instrument or a robotic platform such as the Beckman Coulter Biomek® FX. Magne™ Protein A and Magne™ Protein G Beads:   High purity and rapid, efficient antibody recovery Low nonspecific binding Ease of handling and cost effectiveness High binding capacity1 (≥25mg) of the beads for Protein A and Protein G Optimized performance

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Sample PreparationSample preparation can improve the quality and speed of separation techniques. Products to assist sample preparation include filtration equipment, evaporators, membranes and sieves.AntibodiesAntibodies are used in techniques such as confocal and fluorescence microscopy, flow cytometry, ELISA, ELISPOT, immunohistochemistry, western blotting and immunopreciptation. Select specific antigen reactivity, high specific affinity, low non-specific binding, monoclonal or polyclonal, primary or secondary antibodies and associated conjugates such as an enzyme or dye for visualization.Process ChemistryProcess chemistry is an important stage of drug development for scaling-up drug production or chemical synthesis reactions. It is useful for optimizing economical and efficient drug production. Process chemistry uses reactors and pump systems as well as reagents, standards and buffers.Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Magnetic BeadsFluorescenceThe emission of fluorescence occurs when a photon of energy is supplied to a fluorescent chemical compound by an external source, causing it to become excited. Fluorescence can be detected and measured for different purposes using microplate readers, fluorescence microscopes, fluorescence scanners, and flow cytometers.Antibody PurificationProtein BiologyThe analysis of protein expression, identity and function is vital for many areas of life science research and drug discovery. Some of the most commonly used techniques in protein analysis include Western blotting, electrophoresis and mass spectrometry.ConjugationAntibody Drug Conjugates

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