Development of a New Real-Time PCR Quantitation System for Human and Y DNA Analysis and Normalization Software

5 Nov 2013

This application note discusses the development of a new technology for real-time quantitative PCR. The technology offers an advantage over currently available systems by simultaneously quantitating both the total human DNA and male-specific DNA within a sample, in addition to an internal PCR control. Simultaneous quantification of autosomal and Y-chromosome DNA means: less variability, less time and more valuable data.

Plexor® HY System

Promega Corp.

  If there is DNA to detect, the Plexor® HY System will show it. Total human DNA. Total male DNA. The power to see it all in a single assay.   The Plexor® HY System is a real-time PCR assay to determine the concentration of total human DNA and male human DNA simultaneously in one reaction. The kit contains an internal PCR control (IPC) to test for false-negative results that may occur in the presence of PCR inhibitors and a melt curve function to confirm that the correct product was amplified. Plexor® HY is a sensitive multiplex kit that routinely detects approximately 6.4pg of total DNA. PCR setup is performed at room temperature and is compatible with automated platforms. Plexor® HY DNA Quantitation System Features: Simultaneous quantification of autosomal and Y-chromosomal DNA - Less variability, less time, more valuable data. Consistent and reproducible detection of 6.4pg of DNA - If you can't detect it with Plexor® HY, you can't detect it with your STR system. Internal PCR control and melt-curve analysis - Guard against false negative and false positive results, allowing you to be confident in your data.

(0)

Links

Tags

PCR and Thermal CyclingPolymerase chain reaction (PCR) kits and thermal cyclers are used for the in vitro amplification of DNA permitting subsequent analysis and experimental procedures. Explore a range of high-quality polymerase, primers and nucleotides or simplify your workflow with a PCR mastermix. Find reverse transcription PCR (RT-PCR) and cDNA synthesis kits for RNA products and libraries. Quantitatively measure the amplification of DNA with real-time PCR (qPCR) and droplet digital PCR (ddPCR) kits and systems, and discover automated PCR setup solutions to increase throughput. Alternative DNA amplification methods also include recombinase polymerase amplification (RPA) kits. Find the best PCR kits and thermal cyclers and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.qPCRQuantitative PCR (qPCR) is a molecular biology technique used to amplify and measure the amount of a specific DNA or RNA target. It plays a crucial role in gene expression analysis, pathogen detection, and quantifying DNA or RNA in various applications. Real-time PCR provides precise, quantitative results and is widely used in research, diagnostics, and clinical studies. Browse our peer-reviewed product directory to find the best qPCR solutions; compare products, check reviews, and get pricing directly from manufacturers.DNA AmplificationDNA Amplification is a technique used to amplify a single or multiple copies of DNA or mRNA by orders of magnitude. The most common method employed is PCR, but other options exist that eliminate the need for thermocycling.RT qPCRReal-time quantitative PCR (RT-qPCR) is a technique used to measure gene expression and quantify DNA or RNA in real-time. It is essential in genomics, diagnostics, and biotechnology research. Explore RT-qPCR systems in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.
Development of a New Real-Time PCR Quantitation System for Human and Y DNA Analysis and Normalization Software