ResourceLife Sciences
Development of a Multiplex High Throughput Screening Assay Cascade for Evaluating Receptor Tyrosine Kinase Binding and Activation
24 Sept 2012Screening for protein based therapeutics that inhibit ligand binding and/or downstream activation events in living cells represents a powerful approach for the discovery of novel drugs. This scientific poster, presented at ELRIG Drug Discovery 2012, describes how IntelliCyt have developed a series of live-cell based screening assays for the HTFC® Screening System that addresses multiple steps in these ligand-receptor driven pathways. For these studies, interactions between EphA2 receptor and its ligands were used as a model system. The results demonstrate that high throughput flow cytometry can be leveraged to improve productivity in the drug discovery process.
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High-Throughput ScreeningHigh-throughput screening (HTS) is an automated drug discovery technique for identification of active compounds against a compound library. Use HTS readers and integrated assay preparation / analysis workstations to screen your compounds. Identify active compounds against various HTS libraries, including membranes, proteins and peptides and HTS cell lines. Find the best high-throughput screening products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Flow Cytometry / Cell CountingFlow cytometers are used to count, sort and examine multiple characteristics of cells. Other cell analysis equipment includes image cytometers, cell counters, fluorescence-activated cell sorters (FACS), magnetic-activated cell sorters (MACS), and a range of flow cytometry assay kits. Flow cytometers can reveal information on cell viability, cell proliferation, apoptosis and cell cycle progression, as well as identify cell populations and intracellular or cell-surface molecules. Additionally, some flow cytometers, known as FACS, have an additional sorting function after analysis. Cell counters and image cytometers count live and dead cell populations and can also conduct cell proliferation assays. Find the best flow cytometers, cell counters and cell sorters in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.ELRIGEphrin Receptors