ResourceLife Sciences
Development of a Magnetic Bead-based Multiplex Immunoassay for Metabolic Biomarkers in Normal and Diabetic Mouse Serum and Plasma Samples
16 Mar 2017Obesity and diabetes are on the rise and constitute a series of complex metabolic disorders with complex interactions among multiple proteins and environmental factors. Murine diabetes and obesity models exist to study these disorders through the measurement of circulating metabolic biomarkers, however, the limited amount of sample poses a challenge to effective analysis. This poster describes a novel multiplex immunoassay that can simultaneously measure eight of the most commonly measured metabolic biomarkers, using the Bio-Plex suspension array system.
Related products
Request Quote for All Products
Bio-Plex® 3D Suspension Array System
Bio-RadIncludes Bio-Plex 3D Suspension Array System, xPONENT 4.0 acquisition software, PC, Calibration Reagents, Verification Reagents, Bio-Plex Manager 6.0, Desktop License.
Links
Tags
Cellular PathologyCellular Pathology deals with the microscopic analysis of tissue samples and cells. Sample preparation and processing includes fixation, staining, sectioning and slide mounting, using equipment such microtomes and cryostats. In choosing immunohistochemistry and immunocytochemistry kits, consider chromogens, staining method, antibodies, microscopes and imaging.BiomarkersBiomarkers are biological markers which can be measured and evaluated to indicate a biological state. The use of biomarkers in research and diagnosis can indicate a normal or disease state or drug response of cells / tissues. Biomarkers include genetic markers, cell surface markers such as antigens, antibodies or receptors and secreted molecules such as cytokines. An assay system is required for identification of biomarkers. :Animal ModelsThe use of non-human animals in experiments or behavorial observations. The research is conducted inside universities, medical schools, pharmaceutical companies, farms, defence establishments, and commercial facilities that provide animal-testing services to industry. It includes pure research such as genetics, developmental biology, behavioral studies, as well as applied research such as pharmaceutical testing in pre-clinical, before human, studies. ImmunoassayImmunoassays are laboratory tests that utilize antibody-antigen interactions to detect specific substances. They are widely used in diagnostics for detecting diseases, drug testing, and environmental monitoring. These assays offer rapid, sensitive, and precise detection of biomarkers, making them invaluable in clinical and research labs. Browse our peer-reviewed product directory to compare immunoassay systems, read user reviews, and get pricing directly from manufacturers.DiabetesDiabetes is a metabolic disorder characterized by high blood sugar levels, either due to insufficient insulin production or resistance to its effects. Ongoing diabetes innovation focuses on developing better diagnostic tools, treatments, and preventive measures. Browse our peer-reviewed product directory to find the best diabetes diagnostic tools, compare products, check reviews, and get pricing directly from manufacturers.MultiplexBiomarker DiscoveryClinical biomarkers refer to substances related to known medical conditions that can be accurately measured <i>in vitro</i>. Biomarkers can be used to diagnose presence of a disease and indicate disease severity. The discovery of new biomarkers is incredibly valuable in the field of diagnostics.