ResourceLife Sciences
DNA shearing devices for short- and long-read sequencing
24 Jun 2024One of the main focuses of Hologic Diagenode’s epigenetics department is developing DNA and chromatin shearing devices for short- and long-read sequencing. During DNA sizing and quality analysis, the team opted to use the Agilent Femto Pulse and Fragment Analyzer systems, which can also be utilized for applications such as RNA, chromatin-based methylation studies, and NGS library profiling. Hologic Diagenode explains how its team uses these Agilent systems to achieve higher throughout and greater flexibility in its work, supporting the goal of getting new devices to customers.
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DNA SequencingDNA sequencing, such as sanger sequencing, is a biological technique that determines the precise order of nucleotide bases in a fragment or template of DNA. DNA sequencers and genetic analyzers are based on capillary electrophoresis, where labeled DNA fragments are electrophoretically separated by size as they migrate through a polymer. Find the best DNA sequencing products, including DNA sequencing kits, genomic libraries and genetic identity kits in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Next Generation SequencingNext-generation sequencing (NGS), also known as whole-genome sequencing, high-throughput sequencing and massive parallel sequencing, produces and analyses thousands to millions of nucleotide sequences at once. Sequencing systems operate via varying technologies depending on the manufacturer, including sequencing by synthesis, ligation, pyrosequencing, ion semiconductor and single-molecule real-time sequencing. For NGS, library preparation is paramount to successful sequencing. In this section, explore a range of library preparation kits, from targeted, amplicon-based or hybridization-based kits including epigenomic, transcriptomic and genomic workflows to fragmentation kits. Find the best next-generation sequencing products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.DNA ShearingQuality ControlQuality control is needed in all production processes. Quality control is a monitoring procedure or set of procedures that are put in place to ensure that a manufactured product adheres to a defined set of quality criteria.

